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Rabbit Anti-APOBEC3G Recombinant Antibody (CBYY-C1865) (CBMAB-C3303-YY)


See all APOBEC3G antibodies
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Published Data
Rabbit Anti-APOBEC3G Recombinant Antibody (CBYY-C1865) in WB
Western blot analysis with lysates of control or IRGM+/− HT-29 cells or Flag IRGM complemented IRGM+/− HT-29 cells with indicated antibodies of viral restriction factors. ...View More
Nath, P., Chauhan, N. R., Jena, K. K., Datey, A., Kumar, N. D., Mehto, S., ... & Chauhan, S. (2021). Inhibition of IRGM establishes a robust antiviral immune state to restrict pathogenic viruses. EMBO reports, 22(11), e52948.
Rabbit Anti-APOBEC3G Recombinant Antibody (CBYY-C1865) in Immunoblot
2C reduced the expression of A3G. A3G-HA plus VR1012 (negative control) or the indicated EV71 non-structural proteins were co-transfected into H9 cells. The cells were harvested for immunoblotting analysis at 48 h post-transfection. ...View More
Li, Z., Ning, S., Su, X., Liu, X., Wang, H., Liu, Y., ... & Zhang, W. (2018). Enterovirus 71 antagonizes the inhibition of the host intrinsic antiviral factor A3G. Nucleic Acids Research, 46(21), 11514-11527.

Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBYY-C1865
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Gly255 of human APOBEC3G protein.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Apolipoprotein B MRNA Editing Enzyme Catalytic Subunit 3G
Entrez Gene ID
60489
UniProt ID
P17676
Function
DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against Vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV). May inhibit the mobility of LTR and non-LTR retrotransposons.
Biological Process
Base conversion or substitution editing Source: HGNC-UCL
Cytidine deamination Source: UniProtKB
Cytidine to uridine editing Source: GO_Central
Defense response to virus Source: UniProtKB
DNA cytosine deamination Source: UniProtKB
DNA demethylation Source: GO_Central
Innate immune response Source: HGNC-UCL
Negative regulation of single stranded viral RNA replication via double stranded DNA intermediate Source: UniProtKB
Negative regulation of transposition Source: UniProtKB
Negative regulation of viral genome replication Source: UniProtKB
Negative regulation of viral process Source: HGNC-UCL
Positive regulation of defense response to virus by host Source: HGNC-UCL
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus; Cytoplasm; P-body. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 Vif.
PTM
Ubiquitinated in the presence of HIV-1 Vif. Association with Vif targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway.
Phosphorylation at Thr-32 reduces its binding to HIV-1 Vif and subsequent ubiquitination and degradation thus promoting its antiviral activity.
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For research use only. Not intended for any clinical use.

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