Mouse Anti-APOBEC3G Recombinant Antibody (6C2) (CBMAB-BR032LY)

Name: Mouse Anti-APOBEC3G Recombinant Antibody (6C2)
SKU: CBMAB-BR032LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

6C2

Antibody Isotype

IgG1

Application

FC, IHC-P, WB, IF

Basic Information

Immunogen

E.coli-derived human APOBEC3G recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	0.1-0.5 μg/ml
FC	1-3 μg/10^6 cells
IF(ICC)	2 μg/ml
IHC-P	0.5-1 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

0.05 mg sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Apolipoprotein B MRNA Editing Enzyme Catalytic Subunit 3G

Introduction

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene catalyzes site-specific deamination of both RNA and single-stranded DNA. The encoded protein has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity. [provided by RefSeq, Mar 2017]

Entrez Gene ID

60489

UniProt ID

Q9HC16

Alternative Names

DNA dC->dU-editing enzyme APOBEC-3G; APOBEC-related cytidine deaminase; APOBEC-related protein; ARCD; APOBEC-related protein 9; ARP-9; CEM-15; CEM15; Deoxycytidine deaminase; A3G; APOBEC3G; MDS019

Function

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against Vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV). May inhibit the mobility of LTR and non-LTR retrotransposons.

Biological Process

Base conversion or substitution editing Source: HGNC-UCL
Cytidine deamination Source: UniProtKB
Cytidine to uridine editing Source: GO_Central
Defense response to virus Source: UniProtKB
DNA cytosine deamination Source: UniProtKB
DNA demethylation Source: GO_Central
Innate immune response Source: HGNC-UCL
Negative regulation of single stranded viral RNA replication via double stranded DNA intermediate Source: UniProtKB
Negative regulation of transposition Source: UniProtKB
Negative regulation of viral genome replication Source: UniProtKB
Negative regulation of viral process Source: HGNC-UCL
Positive regulation of defense response to virus by host Source: HGNC-UCL
Viral process Source: UniProtKB-KW

Cellular Location

Nucleus; Cytoplasm; P-body. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 Vif.

PTM

Ubiquitinated in the presence of HIV-1 Vif. Association with Vif targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway.
Phosphorylation at Thr-32 reduces its binding to HIV-1 Vif and subsequent ubiquitination and degradation thus promoting its antiviral activity.

More Infomation

References

Stupfler, B., Verriez, C., Gallois-Montbrun, S., Marquet, R., & Paillart, J. C. (2021). Degradation-Independent Inhibition of APOBEC3G by the HIV-1 Vif Protein. Viruses, 13(4), 617.

Salas Briceno, K., & Ross, S. R. (2021). Repair of APOBEC3G-mutated retroviral DNA in vivo is facilitated by the host enzyme uracil DNA glycosylase 2. Journal of Virology, JVI-01244.

Stupfler, B., Verriez, C., Gallois-Montbrun, S., Marquet, R., & Paillart, J. C. (2021). Degradation-Independent Inhibition of APOBEC3G by the HIV-1 Vif Protein. Viruses 2021, 13, 617.

Botvinnik, A., Shivam, P., Smith, Y., Sharma, G., Olshevsky, U., Moshel, O., ... & Kotler, M. (2021). APOBEC3G rescues cells from the deleterious effects of DNA damage. The FEBS Journal.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Rabbit Anti-APOBEC3G Recombinant Antibody (CBYY-C1865)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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