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Mouse Anti-APAF1 Recombinant Antibody (CBLNA-116) (CBMAB-1085-CN)


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Published Data
Mouse Anti-APAF1 Recombinant Antibody (CBLNA-116) in Immunoblot
Effects of HDACi and HSP90i incubation or preincubation prior to cisplatin on protein expression levels of pro-/antiapoptotic proteins. Representative immunoblot analysis of APAF-1, p21, survivin and α-tubulin (as loading control) in A2780 and A2780CisR cells is shown. For combination treatments, panobinostat (pano) and/or luminespib (lumi) were administrated 48 h prior to 24 h cisplatin treatment with respective IC50 concentrations. Cell line specific control was treated with vehicle for 72 h. ...View More
Rodrigues Moita, A. J., Bandolik, J. J., Hansen, F. K., Kurz, T., Hamacher, A., & Kassack, M. U. (2020). Priming with HDAC inhibitors sensitizes ovarian cancer cells to treatment with cisplatin and HSP90 inhibitors. International Journal of Molecular Sciences, 21(21), 8300.
Mouse Anti-APAF1 Recombinant Antibody (CBLNA-116) in Immunoblot
832/13 and primary rat islets were untreated (no virus) or treated with recombinant adenoviruses containing cDNAs corresponding to human Apaf-1, human caspase 3, human caspase 9 or the control gene, β-galactosidase (β-gal), as indicated. 72 h post-infection, 832/13 cells (A) and primary rat islets (B) were treated with DMSO (control) or thapsigargin (100 nM or 1 μM, respectively) for 18 h. ...View More
Hayes, H. L., Peterson, B. S., Haldeman, J. M., Newgard, C. B., Hohmeier, H. E., & Stephens, S. B. (2017). Delayed apoptosis allows islet β-cells to implement an autophagic mechanism to promote cell survival. PloS one, 12(2), e0172567.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBLNA-116
Antibody Isotype
IgG2b
Application
WB

Basic Information

Immunogen
E. coli-derived recombinant human APAF-1 Leu10-Asp214.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Apoptotic Peptidase Activating Factor 1
Introduction
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Entrez Gene ID
317
UniProt ID
O14727
Alternative Names
CED4; APAF-1
Function
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Biological Process
Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: UniProtKB
Aging Source: Ensembl
Apoptotic process Source: ProtInc
Cardiac muscle cell apoptotic process Source: Ensembl
Cell differentiation Source: Ensembl
Cellular response to transforming growth factor beta stimulus Source: Ensembl
Forebrain development Source: Ensembl
Intrinsic apoptotic signaling pathway Source: UniProtKB
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: Ensembl
Kidney development Source: Ensembl
Negative regulation of G0 to G1 transition Source: Reactome
Nervous system development Source: ProtInc
Neural tube closure Source: Ensembl
Neuron apoptotic process Source: Ensembl
Neutrophil degranulation Source: Reactome
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of apoptotic signaling pathway Source: Ensembl
Regulation of apoptotic DNA fragmentation Source: Ensembl
Regulation of apoptotic process Source: UniProtKB
Response to G1 DNA damage checkpoint signaling Source: UniProtKB
Response to hypoxia Source: Ensembl
Response to nutrient Source: Ensembl
Cellular Location
Cytoplasm
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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