Mouse Anti-ANKS1A Recombinant Antibody (CBXO-0345) (CBMAB-O0317-CQ)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBXO-0345

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 731-808 mapping within an internal region of Odin of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Ankyrin Repeat And Sterile Alpha Motif Domain Containing 1A

Introduction

Regulator of different signaling pathways. Regulates EPHA8 receptor tyrosine kinase signaling to control cell migration and neurite retraction (By similarity).

Entrez Gene ID

Human	23294
Mouse	110338067
Rat	309639

UniProt ID

Human	Q92625
Mouse	P59672
Rat	D4AC12

Alternative Names

Ankyrin Repeat And Sterile Alpha Motif Domain Containing 1A; Ankyrin Repeat And SAM Domain Containing 1; ANKS1; Odin; Ankyrin Repeat And Sterile Alpha Motif Domain Containing 1; Ankyrin Repeat And SAM Domain-Containing Protein 1A; KIAA0229

Function

Regulator of different signaling pathways. Regulates EPHA8 receptor tyrosine kinase signaling to control cell migration and neurite retraction (By similarity).

Biological Process

Ephrin receptor signaling pathway Source: UniProtKB
Neuron remodeling Source: UniProtKB
Regulation of ephrin receptor signaling pathway Source: InterPro
Substrate-dependent cell migration Source: UniProtKB

Cellular Location

Cytoplasm; Cell projection. Cytoplasmic before and after growth factor treatment.

PTM

Phosphorylated on tyrosine residues in response to EGF and PDGF.

More Infomation

References

Fantino, M., Paquette, M., Bernard, S., & Baass, A. (2021). ANKS1A genotype predicts cardiovascular events in patients with familial hypercholesterolemia. Journal of Clinical Lipidology.

Park, S., Lee, H., Lee, J., Park, E., & Park, S. (2019). Ependymal cells require ANKS1A for their proper development. Molecules and cells, 42(3), 245.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

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