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Mouse Anti-ALDH5A1 Recombinant Antibody (17F5) (CBMAB-A2299-YC)


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Published Data
Mouse Anti-ALDH5A1 Recombinant Antibody (17F5) in IHC
SSADH immunoreactive cells are enriched in proliferative/non-differentiated GBM territories (P HIGH/D −) and rare in non-proliferative/differentiated (P LOW/D +) tumor territories of patients' GBM, as revealed by immunohistochemical staining of Ki67, Olig2, and GFAP. HES: hematoxylin and eosin staining. Scale bar 100 µm. ...View More
El-Habr, E. A., Dubois, L. G., Burel-Vandenbos, F., Bogeas, A., Lipecka, J., Turchi, L., ... & Junier, M. P. (2017). A driver role for GABA metabolism in controlling stem and proliferative cell state through GHB production in glioma. Acta neuropathologica, 133, 645-660.
Mouse Anti-ALDH5A1 Recombinant Antibody (17F5) in WB
Decreased SSADH levels in GBM and DIPG stem-like cells (TG1, TP54) upon ALDH5A1 downregulation with siRNAs (siA). siC (control siRNA). Mean ± SD, n = 3 independent biological samples. RDU, relative densitometry units. ...View More
El-Habr, E. A., Dubois, L. G., Burel-Vandenbos, F., Bogeas, A., Lipecka, J., Turchi, L., ... & Junier, M. P. (2017). A driver role for GABA metabolism in controlling stem and proliferative cell state through GHB production in glioma. Acta neuropathologica, 133, 645-660.

Summary

Host Animal
Mouse
Specificity
Human
Clone
17F5
Antibody Isotype
IgG2a, κ
Application
WB, ELISA, ICC, IF, IHC

Basic Information

Immunogen
Recombinant human ALDH5A1 (48-535aa) purified from E. coli
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IF(ICC)1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 10% Glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aldehyde Dehydrogenase 5 Family Member A1
Introduction
ALDH5A1 belongs to the aldehyde dehydrogenase family of proteins. ALDH5A1 is a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of
Entrez Gene ID
7915
UniProt ID
P51649
Alternative Names
Aldehyde Dehydrogenase 5 Family Member A1; NAD(+)-Dependent Succinic Semialdehyde Dehydrogenase; EC 1.2.1.24; SSADH; Succinate-Semialdehyde Dehydrogenase, Mitochondrial; Mitochondrial Succinate Semialdehyde Dehydrogenase;
Function
Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).
Biological Process
Central nervous system development Source: UniProtKB
Gamma-aminobutyric acid catabolic process Source: UniProtKB
Glutamate metabolic process Source: UniProtKB
Neurotransmitter catabolic process Source: UniProtKB
Post-embryonic development Source: Ensembl
Succinate metabolic process Source: UniProtKB
Cellular Location
Mitochondrion
Involvement in disease
Succinic semialdehyde dehydrogenase deficiency (SSADHD): A rare inborn error of 4-aminobutyric acid (GABA) metabolism, which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is clinically characterized by developmental delay, hypotonia, mental retardation, ataxia, seizures, hyperkinetic behavior, aggression, and sleep disturbances.
More Infomation

Yoganathan, S., Arunachal, G., Kratz, L., Varman, M., Thomas, M., Sudhakar, S. V., ... & Danda, S. (2021). Metabolic or ischemic stroke in succinic semi-aldehyde dehydrogenase deficiency due to the homozygous variant c. 1343+ 1_1343+ 3delGTAinsTT in ALDH5A1. Annals of Indian Academy of Neurology, 24(2), 259.

Fattahi, M., Bushehri, A., Alavi, A., Asghariazar, V., Nozari, A., Firouzabadi, S. G., ... & Behjati, F. (2020). Bi-allelic Mutations in ALDH5A1 is associated with succinic semialdehyde dehydrogenase deficiency and severe intellectual disability. Gene, 144918.

Vernau, K. M., Struys, E., Letko, A., Woolard, K. D., Aguilar, M., Brown, E. A., ... & Bannasch, D. L. (2020). A Missense Variant in ALDH5A1 Associated with Canine Succinic Semialdehyde Dehydrogenase Deficiency (SSADHD) in the Saluki Dog. Genes, 11(9), 1033.

Cao, C., Wang, X., Li, R., Jin, P., Chen, H., Xia, M., ... & Tian, X. (2020). Downregulation of ALDH5A1 Promotes Tumor Metastasis and Contributes to Poor Prognosis in Ovarian Cancer.

Menduti, G., Biamino, E., Vittorini, R., Vesco, S., Puccinelli, M. P., Porta, F., ... & Rossi, L. (2018). Succinic semialdehyde dehydrogenase deficiency: The combination of a novel ALDH5A1 gene mutation and a missense SNP strongly affects SSADH enzyme activity and stability. Molecular genetics and metabolism, 124(3), 210-215.

Tian, X., Han, Y., Yu, L., Luo, B., Hu, Z., Li, X., ... & Ma, D. (2017). Decreased expression of ALDH5A1 predicts prognosis in patients with ovarian cancer. Cancer biology & therapy, 18(4), 245-251.

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For research use only. Not intended for any clinical use.

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