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Mouse Anti-AHSG Recombinant Antibody (V2-180308) (CBMAB-A1765-YC)


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Published Data
Mouse Anti-AHSG Recombinant Antibody (CBYC-A451) (CBMAB-A1765-YC) in Immunoblot
Validation immunoblot experiments and densitometric analyses showing protein expression in normal, early-stage, and late-stage myxomatous valves. ...View More
Lacerda, C. M., Disatian, S., & Orton, E. C. (2009). Differential protein expression between normal, early-stage, and late-stage myxomatous mitral valves from dogs. PROTEOMICS-Clinical Applications, 3(12), 1422-1429.
Mouse Anti-AHSG Recombinant Antibody (CBYC-A451) (CBMAB-A1765-YC) in IF
Confirmed the enhanced Fetuin-A in pal-induced insulin-resistant HepG2 cells by immunofluorescence. ...View More
Singh, P., Garg, R., Goand, U. K., Riyazuddin, M., Reza, M. I., Syed, A. A., ... & Gayen, J. R. (2020). Combination of Pancreastatin inhibitor PSTi8 with metformin inhibits Fetuin-A in type 2 diabetic mice. Heliyon, 6(10), e05133.
Mouse Anti-AHSG Recombinant Antibody (CBYC-A451) (CBMAB-A1765-YC) in Immunoblot
Immunoblotting of Fetuin-A in the liver. ...View More
Singh, P., Garg, R., Goand, U. K., Riyazuddin, M., Reza, M. I., Syed, A. A., ... & Gayen, J. R. (2020). Combination of Pancreastatin inhibitor PSTi8 with metformin inhibits Fetuin-A in type 2 diabetic mice. Heliyon, 6(10), e05133.
Mouse Anti-AHSG Recombinant Antibody (CBYC-A451) (CBMAB-A1765-YC) in Immunogold staining
Immunogold staining of mineral particles observed in human arteries of diabetic subjects and specimens prepared in vitro. Human artery tissues (A-O) and HS-particles prepared in vitro (P-T) and processed for thin-section TEM and immunogold labeling as described in the Methods. Gold-NP-coupled antibodies used for these experiments are indicated on top. An antibody that reacts against FenB, a protein from Bacillus subtilis, was used as a negative control. ApoA1, apolipoprotein-A1; FenB, fengycin B; HSA, human serum albumin; HSF, human serum fetuin-A; HS-NPs, human serum-nanoparticles. ...View More
Wu, C. Y., Martel, J., & Young, J. D. (2020). Ectopic calcification and formation of mineralo-organic particles in arteries of diabetic subjects. Scientific Reports, 10(1), 1-10.
Mouse Anti-AHSG Recombinant Antibody (CBYC-A451) (CBMAB-A1765-YC) in IF
Immunohistological staining of artery tissues from diabetic subjects showing colocalization of serum proteins and mineral deposits. Serial tissue sections were processed for albumin and fetuin-A immunofluorescence staining (A-D), von Kossa staining (E-H), and confocal fluorescence (I-L) as described in the Methods. White arrows indicate positive signals for immunofluorescence (albumin and/or fetuin-A) and von Kossa staining (calcification). White rectangles indicate the enlarged areas of the insets. TI, tunica intima; TM, tunica media. ...View More
Wu, C. Y., Martel, J., & Young, J. D. (2020). Ectopic calcification and formation of mineralo-organic particles in arteries of diabetic subjects. Scientific Reports, 10(1), 1-10.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-180308
Antibody Isotype
IgG1
Application
IHC, WB, ELISA, IP

Basic Information

Immunogen
Amino acids 68-367 of fetuin-A of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:100-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Alpha 2-HS Glycoprotein
Introduction
AHSG is a negatively-charged serum glycoprotein that is synthesized by hepatocytes. The encoded protein consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several processes, including
Entrez Gene ID
Human197
Mouse11625
Rat25373
UniProt ID
HumanP02765
MouseP29699
RatP24090
Alternative Names
Alpha 2-HS Glycoprotein; Ba-Alpha-2-Glycoprotein; Alpha-2-Z-Globulin; Fetuin-A; FETUA; Alpha-2-HS-Glycoprotein;
Function
Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
Biological Process
Acute-phase response Source: UniProtKB
Cellular protein metabolic process Source: Reactome
Negative regulation of bone mineralization Source: UniProtKB
Negative regulation of endopeptidase activity Source: GO_Central
Negative regulation of insulin receptor signaling pathway Source: UniProtKB
Neutrophil degranulation Source: Reactome
Pinocytosis Source: UniProtKB
Platelet degranulation Source: Reactome
Positive regulation of phagocytosis Source: UniProtKB
Post-translational protein modification Source: Reactome
Regulation of bone mineralization Source: UniProtKB
Regulation of inflammatory response Source: UniProtKB
Skeletal system development Source: UniProtKB
Cellular Location
Secreted
Involvement in disease
Alopecia-mental retardation syndrome 1 (APMR1): A rare autosomal recessive form of alopecia. APMR1 patients show loss of hair on the scalp, absence of eyebrows, eyelashes, axillary and pubic hair, and mild to severe mental retardation.
PTM
Phosphorylated by FAM20C in the extracellular medium.
O- and N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan at Asn-156: Hex5HexNAc4; N-glycan heterogeneity at Asn-176: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor).
More Infomation

Mancuso, E., Spiga, R., Rubino, M., Averta, C., Rotundo, S., Segura-Garcìa, C., ... & Andreozzi, F. (2021). Effects of Alpha-2-HS-glycoprotein on cognitive and emotional assessment in prediabetic and diabetic subjects. Journal of Affective Disorders, 282, 700-706.

Muzasti, R. A., Purwanto, B., & Sembiring, R. J. (2020). Is there an association between alpha 2-Heremans-Schmid glycoprotein (AHSG) gene Thr256Ser polymorphisms with aortic calcification in regular hemodialysis patients in Medan, Indonesia. Medicinski Glasnik, 17(2).

Roy, D., Fouzder, C., Mukhuty, A., Pal, S., Mondal, M. K., Kundu, R., & Chowdhury, P. (2019). Designed synthesis of dual emitting silicon quantum dot for cell imaging: direct labeling of alpha 2-HS-glycoprotein. Bioconjugate chemistry, 30(5), 1575-1583.

Lin, Y. H., Zhu, J., Meijer, S., Franc, V., & Heck, A. J. (2019). Glycoproteogenomics: A Frequent Gene Polymorphism Affects the Glycosylation Pattern of the Human Serum Fetuin/α-2-HS-Glycoprotein [S]. Molecular & Cellular Proteomics, 18(8), 1479-1490.

Heo, J. I., Yoon, D. W., Yu, J. H., Kim, N. H., Yoo, H. J., Seo, J. A., ... & Kim, N. H. (2018). Melatonin improves insulin resistance and hepatic steatosis through attenuation of alpha‐2‐HS‐glycoprotein. Journal of pineal research, 65(2), e12493.

Majek, P., Pecankova, K., Cermak, J., Gasova, Z., Prochazka, B., & Dyr, J. E. (2017). Alpha-2-HS-glycoprotein plasma level decrease correlates with age in patients with myelodysplastic syndromes. Cancer Biomarkers, 20(4), 637-639.

Kanno, T., Yasutake, K., Tanaka, K., Hadano, S., & Ikeda, J. E. (2017). A novel function of N-linked glycoproteins, alpha-2-HS-glycoprotein and hemopexin: Implications for small molecule compound-mediated neuroprotection. PLoS One, 12(10), e0186227.

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For research use only. Not intended for any clinical use.

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