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Mouse Anti-ADAM33 Recombinant Antibody (V2-179794) (CBMAB-A1156-YC)


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Published Data
Mouse Anti-ADAM33 Recombinant Antibody (A-3) (CBMAB-A1156-YC) in WB
Calcitriol, beclomethasone 17-propionate and montelukast sodium effect on the expression of Vimentin, ADAM33 and MMP-9. (A,B) mRNA expression and (C,D) protein level of ADAM33 and MMP-9 after exposure to calcitriol, beclomethasone 17-propionate, montelukast sodium, LTD4 and TGF-β. The results of experiments (n = 3) are presented in relation to GAPDH or ACTB as mean ± SEM. Western blots are shown for genes, in which the statistical significance has been observed at the mRNA level. Original Western blots can be found at Figure S1. @ p < 0.05, @@ p < 0.01 versus NT group; # p < 0.05, ## p < 0.01 versus T group; $ p < 0.05 versus L group; % p < 0.05, %% p < 0.01 versus LT group; * p < 0.05, ** p < 0.01. One-way analysis of variance (ANOVA) or Kruskal-Wallis test followed by corresponding post hoc was used to determine differences in several groups, and Student's t-test or Welch's t-test was used to determine statistically significant differences between two means. RQ-relative quantification of genes expression normalized to GAPDH, NT-untreated cells, T-cells treated with TGF-β, L-cells treated with LTD4, LT-cells treated with LTD4 and TGF-β, DMT-cells treated with methanol, DMSO and TGF-β, BT-cells treated with beclomethasone 17-propionate and TGF-β, MLT-cells treated with montelukast sodium, LTD4 and TGF-β, MBLT-cells treated with beclomethasone 17-propionate, montelukast sodium, LTD4 and TGF-β, CT-cells treated with calcitriol and TGF-β, CBT-cells treated with calcitriol, beclomethasone 17-propionate and TGF-β, CMLT-cells treated with calcitriol, montelukast sodium, LTD4 and TGF-β, CBMLT-cells treated with calcitriol, beclomethasone 17-propionate, montelukast sodium, LTD4 and TGF-β. ...View More
Sobczak, M., & Pawliczak, R. (2022). Does Vitamin D Work Synergistically with Anti-Asthmatic Drugs in Airway Remodeling?. International Journal of Molecular Sciences, 23(21), 12798.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-179794
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Raised against amino acids 691-813 mapping at the C-terminus of ADAM33 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin.
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ADAM Metallopeptidase Domain 33
Introduction
ADAM33 is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involvi
Entrez Gene ID
80332
UniProt ID
Q9BZ11
Alternative Names
ADAM Metallopeptidase Domain 33; A Disintegrin And Metalloproteinase Domain 33; C20orf153; ADAM 33; Disintegrin And Metalloproteinase Domain-Containing Protein 33; Disintegrin And Reprolysin Metalloproteinase Family Protein;
Biological Process
Proteolysis
Cellular Location
Membrane
Involvement in disease
Asthma (ASTHMA): The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with wheezing due to spasmodic contraction of the bronchi.
Topology
Extracellular: 30-701 aa
Helical: 702-722 aa
Cytoplasmic: 723-813 aa
PTM
The precursor is cleaved by a furin endopeptidase.
More Infomation

Mamoor, S. (2021). Differential expression of ADAM33 in cancers of the breast.

Zihlif, M., Imraish, A., Al-Rawashdeh, B., Qteish, A., Husami, R., Husami, R., ... & Lee, S. J. (2021). The Association of IgE Levels with ADAM33 Genetic Polymorphisms among Asthmatic Patients. Journal of personalized medicine, 11(5), 329.

Yan, F., Hao, Y., Gong, X., Sun, H., Ding, J., & Wang, J. (2021). Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling. Molecular Medicine Reports, 24(1), 1-12.

Ghani, M. U., Sabar, M. F., Iqbal Bano, M. S., Akram, M., Ifrah Khalid, A. M., & Khan, M. U. (2019). Evaluation of ADAM33 gene’s single nucleotide polymorphism variants against asthma and the unique pattern of inheritance in Northern and Central Punjab, Pakistan. Saudi medical journal, 40(8), 774.

Li, H. F., Yan, L. P., Wang, K., Li, X. T., Liu, H. X., & Tan, W. (2019). Association between ADAM33 polymorphisms and asthma risk: a systematic review and meta-analysis. Respiratory research, 20(1), 1-18.

Ghaemi, M. R., Hemmati, S., Rezaei, A., Sadr, M., Mohebbi, B., Ghaffaripour, H., ... & Mahdaviani, S. A. (2019). Association of ADAM33 T1 Polymorphism With Subgroups of Pediatric Asthma Patients in Iran. Acta Medica Iranica, 635-639.

Fang, L., Wu, J., Huang, T., Zhang, P., Xin, X., & Shi, Y. (2018). TGF-β1 stimulates epithelial‑mesenchymal transition mediated by ADAM33. Experimental and therapeutic medicine, 15(1), 985-992.

Yang, P. J., Hou, M. F., Tsai, E. M., Liang, S. S., Chiu, C. C., Ou‑Yang, F., ... & Wang, T. N. (2018). Breast cancer is associated with methylation and expression of the a disintegrin and metalloproteinase domain 33 (ADAM33) gene affected by endocrine‑disrupting chemicals. Oncology reports, 40(5), 2766-2777.

Sun, F. J., Zou, L. Y., Tong, D. M., Lu, X. Y., Li, J., & Deng, C. B. (2017). Association between ADAM metallopeptidase domain 33 gene polymorphism and risk of childhood asthma: a meta-analysis. Brazilian Journal of Medical and Biological Research, 50.

Deng, R., Zhao, F., & Zhong, X. (2017). T1 polymorphism in a disintegrin and metalloproteinase 33 (ADAM33) gene may contribute to the risk of childhood asthma in Asians. Inflammation Research, 66(5).

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For research use only. Not intended for any clinical use.

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