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Rat Anti-ACE2 Recombinant Antibody (V2-179279) (CBMAB-A0552-YC)


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Summary

Host Animal
Rat
Specificity
Mouse
Clone
V2-179279
Antibody Isotype
IgG2b
Application
WB

Basic Information

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse ACE-2, Gln18-Thr740 (predicted).
Specificity
Mouse
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB0.5 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, trehalose
Preservative
None
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Angiotensin I Converting Enzyme 2
Introduction
ACE2 belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angi
Entrez Gene ID
70008
UniProt ID
Q8R0I0
Alternative Names
Angiotensin I Converting Enzyme 2; Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 2; Angiotensin-Converting Enzyme Homolog; ACE-Related Carboxypeptidase; Metalloprotease MPROT15; Peptidyl-Dipeptidase A;
Function
Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency. In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity.
Isoform 2: Non-functional as a carboxypeptidase. Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63.
Isoform 2: Non-functional as a receptor for human coronavirus SARS-CoV-2.
Biological Process
Angiotensin maturation
Angiotensin-mediated drinking behavior
Blood vessel diameter maintenance
Negative regulation of signaling receptor activity
Positive regulation of amino acid transport
Positive regulation of cardiac muscle contraction
Positive regulation of gap junction assembly
Positive regulation of L-proline import across plasma membrane
Positive regulation of reactive oxygen species metabolic process
Proteolysis
Receptor-mediated virion attachment to host cell
Regulation of cardiac conduction
Regulation of cell population proliferation
Regulation of cytokine production
Regulation of inflammatory response
Regulation of systemic arterial blood pressure by renin-angiotensin
Regulation of transmembrane transporter activity
Regulation of vasoconstriction
Tryptophan transport
Viral entry into host cell
Cellular Location
Secreted; Cell membrane; Apical cell membrane; Cytoplasm; Cilium. Detected in both cell membrane and cytoplasm in neurons.
Isoform 2: Apical cell membrane
Topology
Extracellular: 18-740 aa
Helical: 741-761 aa
Cytoplasmic: 762-805 aa
PTM
N-glycosylation on Asn-90 may limit SARS infectivity.
Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1.
Phosphorylated. Phosphorylation at Tyr-781 probably inhibits interaction with AP2M1 and enables interactions with proteins containing SH2 domains.
More Infomation

Batlle, D., Wysocki, J., & Satchell, K. (2020). Soluble angiotensin-converting enzyme 2: a potential approach for coronavirus infection therapy?. Clinical science, 134(5), 543-545.

Ni, W., Yang, X., Yang, D., Bao, J., Li, R., Xiao, Y., ... & Gao, Z. (2020). Role of angiotensin-converting enzyme 2 (ACE2) in COVID-19. Critical Care, 24(1), 1-10.

South, A. M., Brady, T. M., & Flynn, J. T. (2020). ACE2 (Angiotensin-Converting Enzyme 2), COVID-19, and ACE Inhibitor and Ang II (Angiotensin II) receptor blocker use during the pandemic: The pediatric perspective. Hypertension, 76(1), 16-22.

Serfozo, P., Wysocki, J., Gulua, G., Schulze, A., Ye, M., Liu, P., ... & Batlle, D. (2020). Ang II (angiotensin II) conversion to angiotensin-(1-7) in the circulation is POP (prolyloligopeptidase)-dependent and ACE2 (angiotensin-converting enzyme 2)-independent. Hypertension, 75(1), 173-182.

Zhang, H., Penninger, J. M., Li, Y., Zhong, N., & Slutsky, A. S. (2020). Angiotensin-converting enzyme 2 (ACE2) as a SARS-CoV-2 receptor: molecular mechanisms and potential therapeutic target. Intensive care medicine, 46(4), 586-590.

Bourgonje, A. R., Abdulle, A. E., Timens, W., Hillebrands, J. L., Navis, G. J., Gordijn, S. J., ... & van Goor, H. (2020). Angiotensin‐converting enzyme 2 (ACE2), SARS‐CoV‐2 and the pathophysiology of coronavirus disease 2019 (COVID‐19). The Journal of pathology, 251(3), 228-248.

Cheng, H., Wang, Y., & Wang, G. Q. (2020). Organ‐protective effect of angiotensin‐converting enzyme 2 and its effect on the prognosis of COVID‐19. Journal of medical virology, 92(7), 726-730.

Wang, K., Gheblawi, M., & Oudit, G. Y. (2020). Angiotensin converting enzyme 2: a double-edged sword. Circulation, 142(5), 426-428.

Gheblawi, M., Wang, K., Viveiros, A., Nguyen, Q., Zhong, J. C., Turner, A. J., ... & Oudit, G. Y. (2020). Angiotensin-converting enzyme 2: SARS-CoV-2 receptor and regulator of the renin-angiotensin system: celebrating the 20th anniversary of the discovery of ACE2. Circulation research, 126(10), 1456-1474.

Wang, J., Chen, S., & Bihl, J. (2020). Exosome-mediated transfer of ACE2 (angiotensin-converting enzyme 2) from endothelial progenitor cells promotes survival and function of endothelial cell. Oxidative medicine and cellular longevity, 2020.

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For research use only. Not intended for any clinical use.

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