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Rabbit Anti-ABI1 Recombinant Antibody (V2-367116) (CBMAB-S4070-CQ)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
V2-367116
Antibody Isotype
IgG
Application
WB, IP, ICC, FC

Basic Information

Immunogen
Synthetic peptide.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:10,000-1:50,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Buffer
9.85% Tris glycine, 0.1% BSA, 40% Glycerol
Preservative
0.05% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
abl interactor 1
Introduction
This gene encodes a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interactions with Abelson tyrosine kinases. The encoded protein plays a role in macropinocytosis as a component of the WAVE2 complex, and also forms a complex with EPS8 and SOS1 that mediates signal transduction from Ras to Rac. This gene may play a role in the progression of several malignancies including melanoma, colon cancer and breast cancer, and a t(10;11) chromosomal translocation involving this gene and the MLL gene has been associated with acute myeloid leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 14.
Entrez Gene ID
10006
UniProt ID
Q8IZP0
Alternative Names
Abl Interactor 1; Spectrin SH3 Domain-Binding Protein 1; Eps8 SH3 Domain-Binding Protein; Abl-Binding Protein 4; Abelson Interactor 1; SSH3BP1; ABLBP4; NAP1BP; ABI-1; E3B1;
Function
May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.
Biological Process
Actin polymerization or depolymerization
Fc-gamma receptor signaling pathway involved in phagocytosis
Negative regulation of cell population proliferation
Peptidyl-tyrosine phosphorylation
Transmembrane receptor protein tyrosine kinase signaling pathway
Vascular endothelial growth factor receptor signaling pathway
Viral process
Cellular Location
Cytoskeleton; Nucleus; Cytoplasm; Lamellipodium; Filopodium; Growth cone; Postsynaptic density. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. May shuttle from the postsynaptic densities to the nucleus (By similarity).
Involvement in disease
A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with KMT2A/MLL1. ABI1 isoform 2 was found to be present in acute leukemia KMT2A/MLL1-ABI1 fusion transcript.
PTM
Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. Seems to be phosphorylated at Tyr-53 by ABL1, required for nuclear but not for synaptic localization.
More Infomation

Li, K., Peng, Y. F., Guo, J. Z., Li, M., Zhang, Y., Chen, J. Y., ... & Yu, W. D. (2021). Abelson interactor 1 splice isoform-L plays an anti-oncogenic role in colorectal carcinoma through interactions with WAVE2 and full-length Abelson interactor 1. World Journal of Gastroenterology, 27(15), 1595.

Zhang, Y., Zhong, Z., Li, M., Chen, J., Lin, T., Sun, J., ... & Yu, W. (2021). The roles and prognostic significance of ABI1-TSV-11 expression in patients with left-sided colorectal cancer. Scientific reports, 11(1), 1-15.

Faulkner, J., Jiang, P., Farris, D., Walker, R., & Dai, Z. (2020). CRISPR/CAS9-mediated knockout of Abi1 inhibits p185 Bcr-Abl-induced leukemogenesis and signal transduction to ERK and PI3K/Akt pathways. Jour

Wang, R., Liao, G., Wang, Y., & Tang, D. D. (2020). Distinctive roles of Abi1 in regulating actin-associated proteins during human smooth muscle cell migration. Scientific reports, 10(1), 1-13.

Qi, Y., Liu, J., Chao, J., Scheuerman, M. P., Rahimi, S. A., Lee, L. Y., & Li, S. (2020). PTEN suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating Abi1. Scientific reports, 10(1), 1-13.

Zhou, X., Li, C. H., He, P., Wu, L. F., Lu, X., Lei, S. F., & Deng, F. Y. (2019). Abl interactor 1: A novel biomarker for osteoporosis in Chinese elderly men. Journal of proteomics, 207, 103440.

Chorzalska, A., Morgan, J., Ahsan, N., Treaba, D. O., Olszewski, A. J., Petersen, M., ... & Dubielecka, P. M. (2018). Bone marrow–specific loss of ABI1 induces myeloproliferative neoplasm with features resembling human myelofibrosis. Blood, The Journal of the American Society of Hematology, 132(19), 2053-2066.

Jiang, P., Tang, H., Hogan, H., Tate, S., Ryan, W., Paul, L., ... & Zonghan, D. (2017). Abstract P6-01-14: Dissection of the Abl interactor 1 signaling in metastatic breast cancer cells.

Wang, J. L., Yan, T. T., Long, C., & Cai, W. W. (2017). Oncogenic function and prognostic significance of Abelson interactor 1 in hepatocellular carcinoma. International journal of oncology, 50(5), 1889-1898.

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For research use only. Not intended for any clinical use.

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