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Mouse Anti-ABI1 Recombinant Antibody (V2-179101) (CBMAB-A0342-YC)


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Published Data
Mouse Anti-ABI1 Recombinant Antibody (C-1) (CBMAB-A0342-YC) in WB
Western blots showing protein expressions of ABI1 in Jurkat T cells. ...View More
Lim, S. P., Ioannou, N., Ramsay, A. G., Darling, D., Gäken, J., & Mufti, G. J. (2018). miR-181c-BRK1 axis plays a key role in actin cytoskeleton-dependent T cell function. Journal of leukocyte biology, 103(5), 855-866.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-179101
Antibody Isotype
IgG
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Raised against amino acids 271-350 mapping within an internal region of Abi-1 of human origin
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
abl interactor 1
Introduction
ABI1 is a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interactions with A
Entrez Gene ID
Human10006
Mouse11308
Rat79249
UniProt ID
HumanQ8IZP0
MouseQ8CBW3
RatQ9QZM5
Alternative Names
Abl Interactor 1; Spectrin SH3 Domain-Binding Protein 1; Eps8 SH3 Domain-Binding Protein; Abl-Binding Protein 4; Abelson Interactor 1; SSH3BP1; ABLBP4; NAP1BP; ABI-1; E3B1;
Function
May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.
Biological Process
Actin polymerization or depolymerization
Fc-gamma receptor signaling pathway involved in phagocytosis
Negative regulation of cell population proliferation
Peptidyl-tyrosine phosphorylation
Transmembrane receptor protein tyrosine kinase signaling pathway
Vascular endothelial growth factor receptor signaling pathway
Viral process
Cellular Location
Cytoskeleton; Nucleus; Cytoplasm; Lamellipodium; Filopodium; Growth cone; Postsynaptic density. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. May shuttle from the postsynaptic densities to the nucleus (By similarity).
Involvement in disease
A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with KMT2A/MLL1. ABI1 isoform 2 was found to be present in acute leukemia KMT2A/MLL1-ABI1 fusion transcript.
PTM
Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. Seems to be phosphorylated at Tyr-53 by ABL1, required for nuclear but not for synaptic localization.
More Infomation

Li, K., Peng, Y. F., Guo, J. Z., Li, M., Zhang, Y., Chen, J. Y., ... & Yu, W. D. (2021). Abelson interactor 1 splice isoform-L plays an anti-oncogenic role in colorectal carcinoma through interactions with WAVE2 and full-length Abelson interactor 1. World Journal of Gastroenterology, 27(15), 1595.

Zhang, Y., Zhong, Z., Li, M., Chen, J., Lin, T., Sun, J., ... & Yu, W. (2021). The roles and prognostic significance of ABI1-TSV-11 expression in patients with left-sided colorectal cancer. Scientific reports, 11(1), 1-15.

Faulkner, J., Jiang, P., Farris, D., Walker, R., & Dai, Z. (2020). CRISPR/CAS9-mediated knockout of Abi1 inhibits p185 Bcr-Abl-induced leukemogenesis and signal transduction to ERK and PI3K/Akt pathways. Jour

Wang, R., Liao, G., Wang, Y., & Tang, D. D. (2020). Distinctive roles of Abi1 in regulating actin-associated proteins during human smooth muscle cell migration. Scientific reports, 10(1), 1-13.

Qi, Y., Liu, J., Chao, J., Scheuerman, M. P., Rahimi, S. A., Lee, L. Y., & Li, S. (2020). PTEN suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating Abi1. Scientific reports, 10(1), 1-13.

Zhou, X., Li, C. H., He, P., Wu, L. F., Lu, X., Lei, S. F., & Deng, F. Y. (2019). Abl interactor 1: A novel biomarker for osteoporosis in Chinese elderly men. Journal of proteomics, 207, 103440.

Chorzalska, A., Morgan, J., Ahsan, N., Treaba, D. O., Olszewski, A. J., Petersen, M., ... & Dubielecka, P. M. (2018). Bone marrow–specific loss of ABI1 induces myeloproliferative neoplasm with features resembling human myelofibrosis. Blood, The Journal of the American Society of Hematology, 132(19), 2053-2066.

Jiang, P., Tang, H., Hogan, H., Tate, S., Ryan, W., Paul, L., ... & Zonghan, D. (2017). Abstract P6-01-14: Dissection of the Abl interactor 1 signaling in metastatic breast cancer cells.

Wang, J. L., Yan, T. T., Long, C., & Cai, W. W. (2017). Oncogenic function and prognostic significance of Abelson interactor 1 in hepatocellular carcinoma. International journal of oncology, 50(5), 1889-1898.

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For research use only. Not intended for any clinical use.

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