Rabbit Anti-ABCC3 Recombinant Antibody (V2-179014) (CBMAB-A0249-YC)

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Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

V2-179014

Antibody Isotype

IgG

Application

WB, IP

Basic Information

Immunogen

Recombinant protein surrounding Gly870 of human MRP3 protein.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

ATP BINDING CASSETTE SUBFAMILY C MEMBER 3

Introduction

ABCC3 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN

Entrez Gene ID

8714

UniProt ID

O15438

Alternative Names

ATP Binding Cassette Subfamily C Member 3; ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 3; Canalicular Multispecific Organic Anion Transporter 2; Multi-Specific Organic Anion Transporter D; ATP-Binding Cassette Sub-Family C Member 3; Multidrug Re

Function

ATP-dependent transporter of the ATP-binding cassette (ABC) family that bind and hydrolyze ATP to enable active transport of various substrates including many drugs, toxicants and endogenous compound across cell membranes. Transports glucuronide conjugates such as bilirubin diglucuronide, estradiol-17-beta-o-glucuronide and GSH conjugates such as leukotriene C4 (LTC4). Transports also various bile salts (taurocholate, glycocholate, taurochenodeoxycholate-3-sulfate, taurolithocholate- 3-sulfate) (By similarity). Does not contribute substantially to bile salt physiology but provides an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (By similarity). Can confers resistance to various anticancer drugs, methotrexate, tenoposide and etoposide, by decreasing accumulation of these drugs in cells.

Biological Process

Bile acid and bile salt transport
Canalicular bile acid transport
Drug transmembrane transport
Leukotriene transport
Transmembrane transport
Transport across blood-brain barrier
Xenobiotic transport

Cellular Location

Basolateral cell membrane

Topology

Extracellular: 1-32 aa
Helical: 33-53 aa
Cytoplasmic: 54-73 aa
Helical: 74-94 aa
Extracellular: 95-99 aa
Helical: 100-120 aa
Cytoplasmic: 121-132 aa
Helical: 133-153 aa
Extracellular: 154-171 aa
Helical: 172-192 aa
Cytoplasmic: 193-302 aa
Helical: 303-323 aa
Extracellular: 324-349 aa
Helical: 350-370 aa
Cytoplasmic: 371-426 aa
Helical: 427-447 aa
Extracellular: 448-450 aa
Helical: 451-471 aa
Cytoplasmic: 472-533 aa
Helical: 534-554 aa
Extracellular: 555-576 aa
Helical: 577-597 aa
Cytoplasmic: 598-963 aa
Helical: 964-984 aa
Extracellular: 985-1021 aa
Helical: 1022-1042 aa
Cytoplasmic: 1043-1085 aa
Helical: 1086-1106 aa
Extracellular: 1107 aa
Helical: 1108-1128 aa
Cytoplasmic: 1129-1199 aa
Helical: 1200-1220 aa
Extracellular: 1221-1222 aa
Helical: 1223-1243 aa
Cytoplasmic: 1244-1527 aa

More Infomation

References

Sun, Z., Qi, X., & Zhang, Y. (2020). Bioinformatics Analysis of the Expression of ATP binding cassette subfamily C member 3 (ABCC3) in Human Glioma. Open Medicine, 15(1), 107-113.

Liu, Z., Fu, S., Ma, X., Baxter, S. W., Vasseur, L., Xiong, L., ... & You, M. (2020). Resistance to Bacillus thuringiensis Cry1Ac toxin requires mutations in two Plutella xylostella ATP-binding cassette transporter paralogs. PLoS pathogens, 16(8), e1008697.

Liu, X., Huang, Z., He, Z., Meng, Q., Wang, X., & Hu, Y. (2019). A study of natural IgG antibodies against ATP-binding cassette subfamily C member 3 in oral squamous cell carcinoma. Journal of cancer research and therapeutics, 15(4), 921.

Mesci, S., Marakli, S., Yazgan, B., & YILDIRIM, T. (2019). The effect of ATP-binding cassette (ABC) transporters in human cancers. International Journal of Science Letters, 1(1), 14-19.

Mao, X., He, Z., Zhou, F., Huang, Y., & Zhu, G. (2019). Prognostic significance and molecular mechanisms of adenosine triphosphate-binding cassette subfamily C members in gastric cancer. Medicine, 98(50).

Han, S. H., Kim, J. W., Kim, M., Kim, J. H., Lee, K. W., Kim, B. H., ... & Shin, E. (2019). Prognostic implication of ABC transporters and cancer stem cell markers in patients with stage III colon cancer receiving adjuvant FOLFOX‑4 chemotherapy. Oncology letters, 17(6), 5572-5580.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-ABCC3 Recombinant Antibody (V2-179015)

Mouse Anti-ABCC3 Recombinant Antibody (V2-179016)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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