Mouse Anti-AADAT Recombinant Antibody (V2-51866) (CBMAB-K0247-LY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-51866

Antibody Isotype

IgG2a, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Specific for an epitope mapping between amino acids 158-183 within an internal region of KAT II of human origin

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
ELISA	1:30-1:3,000
IF(ICC)	1:50-1:500
IP	1-2 µl per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Aminoadipate Aminotransferase

Introduction

This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Entrez Gene ID

51166

Alternative Names

Aminoadipate Aminotransferase; Kynurenine Aminotransferase II; L Kynurenine/Alpha Aminoadipate Aminotransferase; Kynurenine--Oxoglutarate Aminotransferase II; Kynurenine--Oxoglutarate Transaminase II; Kynurenine--Oxoglutarate Transaminase 2; Alpha-Aminoadipate Aminotransferase; 2-Aminoadipate Aminotransferase; 2-Aminoadipate Transaminase;

Function

Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).

Biological Process

2-oxoglutarate metabolic process
Alpha-amino acid metabolic process
Biosynthetic process
Glutamate metabolic process
Kynurenine metabolic process
L-lysine catabolic process to acetyl-CoA via saccharopine
Lysine catabolic process
Tryptophan catabolic process

Cellular Location

Mitochondrion

More Infomation

References

Hsu, C. C., Yang, A. Y. P., Chen, J. Y., Tsai, H. H., Lin, S. H., Tai, P. C., ... & Yang, J. C. H. (2021). Lysine Deprivation Induces AKT-AADAT Signaling and Overcomes EGFR-TKIs Resistance in EGFR-Mutant Non-Small Cell Lung Cancer Cells. Cancers, 13(2), 272.

Higazi, A. M., Kamel, H. M., Abdel-Naeem, E. A., Abdullah, N. M., Mahrous, D. M., & Osman, A. M. (2021). Expression analysis of selected genes involved in tryptophan metabolic pathways in Egyptian children with Autism Spectrum Disorder and learning disabilities. Scientific Reports, 11(1), 1-12.

Kocki, T., Wicha-Komsta, K., Zakrocka, I., Pabiś, S., & Turski, W. A. (2019). The 14-day carbamazepine treatment increases expression of KAT1 (Ccbl1) and KAT2 (Aadat) genes in rat brain cortex. European Neuropsychopharmacology, 29, S458-S459.

Teumer, A., Chaker, L., Groeneweg, S., Li, Y., Di Munno, C., Barbieri, C., ... & Medici, M. (2018). Genome-wide analyses identify a role for SLC17A4 and AADAT in thyroid hormone regulation. Nature communications, 9(1), 1-14.

Towfigh, A. (2019). Der Einfluss genetischer Polymorphismen im AADAT-Gen auf die Schizophrenie (Doctoral dissertation, lmu).

Wigner, P., Czarny, P., Synowiec, E., Bijak, M., Talarowska, M., Galecki, P., ... & Sliwinski, T. (2018). Variation of genes encoding KAT1, AADAT and IDO1 as a potential risk of depression development. European Psychiatry, 52, 95-103.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-AADAT Recombinant Antibody (V2-12484)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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