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Human Adipokine Antibody Array (Membrane, 182 Targets) (Y0019) (AbAr-0019-YC)

Specifications

Target Array
Human Adipokine Array
Target
ACE/CD43, ACE-2, ACTH, ADFP, Adiponectin, Adipsin (Factor D), AgRP, AMPKa1, Amylin, Angiopoietin 1, Angiopoietin 2, Angiotensinogen/Angiotensin II, Ang-like Factor, ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4, Apelin Receptor, ApoB, ApoE, Axl, BDNF, bFGF, BMP-2, BMP-3, BMP-3b/GDF-10, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-15, BMPR-IA/ALK-3, BMPR-IB/ALK-6, BMPR-II, b-NGF, C3a des Arg, CART, CD137 (4-1BB), CD36, Clusterin, CNTF, C-peptide, CRP, Cystatin C, Dtk, EGF, EGFR, ENA-78, Endophin Beta, Epiregulin, E-selectin, ET-1 (Endothelin), FABP4, FAM3B, FAS/Apo-1, FGF-10, FGF-6, FSH, Galectin -1, Growth Hormone (Growth Hormone), Ghrelin, GITR, GITR Ligand, GLP-1, Glucagon, Glut1, Glut2, Glut3, Glut5, Glutathione peroxidase 1, Glutathione peroxidase 3, GRO alpha, HCC4, HGF, HSD-1, ICAM1, IFN-gamma, IGF-1, IGF-1 sR, IGFBP-1, IGFBP-2, IGFBP-3, IGF-2, IL-1 R1, IL-1 R4, IL-1a, IL-1b, IL-1 ra, IL-6, IL-6 sR, IL-8, IL-10, IL-11, IL-12, IL-25/IL-17E, INSL3, INSRR, Insulin, Insulin R (CD220), Leptin, Leptin R, LH (Luteinizing Hormone), LIF, LOX, Lymphotactin, MCP-1, MCP-3, M-CSF, MIF, MIP-1 alpha, MIP-1 beta, MIP-3b, MMP-2, MMP-9, MMP-11, MMP-19, MSHa, MSPa, Myostatin, NAIP, NeuroD1, Neurophilin-2, NGF R, NPY (Neuropeptide Y), Obestatin R (GPR-39), Orexin A, Orexin B, OSM, Osteocalcin, Osteonectin, Osteoprotegerin, PARC, PDGF, PDGF-AA, PDGF-AB, PDGF-C, PDGF-D, PEDF, Pentraxin-3, PPARg2/NRIC3, Pref-1, Prohibitin, Prolactin, PYY, RANTES, RBP4, RELMb, Resistin, S100, S100 A8+A9, S100 A10, SAA, SDF-1, SEMA3A, Serotonin, Syndecan-3, TACE, TDAG51, TECK, TGF alpha, TGF-b, Thrombospondin 1, Thrombospondin 2, Thrombospondin 4, TIMP-1, TIMP-2, TIMP-3, TIMP-4, Tissue factor (CD142), TLR2, TLR4, TNF alpha, sTNFRI, sTNFRII, TSG-6, TSH, Vaspin, VCAM-1, VEGF-A, Visfatin/PBEF1, XEDAR
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Label-based
Protocol Outline
Dialyze samples in PBS
Biotinylate Samples
Dialyze biotinylated Samples in PBS
Block array membranes
Incubate with Samples
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
The main adipokines include leptin, adiponectin, resistin, tumor-necrosis factor, interleukin 6, chemokine (C–C motif) ligand 2, interleukin 10, and transforming growth factor-β. The imbalance between pro- and anti-inflammatory adipokines on adipose tissue results in insulin resistance and the development of metabolic syndrome, type 2 diabetes, and cardiovascular disease.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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