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Human Soluble Receptor Antibody Array (Membrane, 105 Targets) (Y0251) (AbAr-0251-YC)


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Specifications

Target Array
Soluble Receptor Array
Target
ACE, HB-EGF, Hematopoeitic Array, ADAM8, ICAM-2/CD102, CD5, Integrin alpha 5/CD49e, ADAM9, IL-1 RII, CD6, Integrin alpha 6/CD49f, ADAM10, IL-15 R alpha, CD30, Integrin alpha 9, ALCAM/CD166, Integrin beta 1/CD29, CD40, Integrin alpha E/CD103, Amphiregulin, Integrin beta 2/CD18, CD43, Integrin alpha L/CD11a, APP (pan), Integrin beta 3/CD61, CD48/SLAMF2, Integrin alpha M/CD11b, BACE-1, Integrin beta 4/CD104, CD59, Integrin alpha X/CD11c, BCAM/CD239, Integrin beta 5, CD84/SLAMF5, LAG-3, C1q R1/CD93, Integrin beta 6, CD97, MMP-9 (total), CD9, JAM-A/CD321, CD163, MMR, CD23/Fc epsilon RII, NGAL, CD229/SLAMF3, Myeloperoxidase, PECAM-1, LOX-1/SR-E1/CLEC8A, Chitinase 3-like 1/YKL40, Resistin, CD36/SR-B3, MD-1/LY86, CRP, L-Selectin, CD40 Ligand, MMP-2 (total), CRTAM, Siglec-5, CD44H, NCAM-1/CD56, CXCL16, Siglec-6, CD58/LFA-3, NCAM-L1/CD171, DNAM-1, Siglec-7, CD90/Thy1, Osteopontin, DPPIV, Siglec-9, CD99, PAR1, IFN-gamma R2, Siglec-10, CD155/PVR, Pref-1/DLK-1/FA1, IL-2 R alpha, TIM-3/KIM-3, CEACAM-1/CD66a, RECK, IL-2 R beta, TLR2/CD282, CX3CL1/Fractalkine, Stabilin-1, IL-6 R, TLR4/CD284, CXCL8, TACE/ADAM17, Integrin alpha 3/CD49c, TNF RI, EMMPRIN/CD147, Thrombospondin-1, Integrin alpha 4/CD49d, TRACP/PAP/ACP5, Endoglin, TIMP-1, TRANCE/RANKL, Epiregulin, TIMP-2, TREM-1, LGALS1, TIMP-3, LGALS3, TNF RII, LGALS3BP/MAC-2BP
Species Reactivity
Human

Applications

Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Apply a blocking agent to the membranes.
Proceed with sample incubation.
Incubate using the cocktail of biotinylated detection antibodies.
Incubate with streptavidin conjugated to horseradish peroxidase (HRP).
Conduct incubation with the detection buffers.
Utilize a chemiluminescent imaging system for image capture.
Execute densitometric evaluation and perform data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
In biochemistry and pharmacology, receptors are chemical structures, composed of protein, that receive and transduce signals that may be integrated into biological systems. These signals are typically chemical messengers, which bind to a receptor, they cause some form of cellular/tissue response, e.g. a change in the electrical activity of a cell. There are three main ways the action of the receptor can be classified: relay of signal, amplification, or integration. Relaying sends the signal onward, amplification increases the effect of a single ligand, and integration allows the signal to be incorporated into another biochemical pathway. In this sense, a receptor is a protein-molecule that recognizes and responds to endogenous chemical signals. For example, an acetylcholine receptor recognizes and responds to its endogenous ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug targets, such as enzymes, transporters, and ion channels.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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