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Human Receptor Antibody Array (Membrane, 40 Targets) (Y0166) (AbAr-0166-YC)


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Specifications

Target Array
Receptor Array
Target
4-1BB (TNFRSF9/CD137), CD30 (TNFRSF8), Endoglin (CD105), GITR (TNFRSF18), IL-10 R beta, Lipocalin-2 (NGAL), NRG1-beta 1 (HRG1-beta 1), TRAIL R3 (TNFRSF10C), ALCAM (CD166), CD40 Ligand (TNFSF5), ErbB3, HVEM (TNFRSF14), IL-17 RA, L-Selectin (CD62L), PDGF R beta, Trappin-2, CD80 (B7-1), CEACAM-1, E-Selectin, ICAM-3 (CD50), IL-2 R gamma (Common gamma Chain), LYVE-1, PECAM-1 (CD31), uPAR, BCMA (TNFRSF17), DR6 (TNFRSF21), Fas (TNFRSF6/Apo-1), IL-1 R4 (ST2), IL-21 R, MICA, RAGE, VCAM-1 (CD106), CD14, Dtk, Flt-3 Ligand, IL-1 R1, LIMPII, MICB, TIM-1 (KIM-1), XEDAR
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
In biochemistry and pharmacology, receptors are chemical structures, composed of protein, that receive and transduce signals that may be integrated into biological systems. These signals are typically chemical messengers, which bind to a receptor, they cause some form of cellular/tissue response, e.g. a change in the electrical activity of a cell. There are three main ways the action of the receptor can be classified: relay of signal, amplification, or integration. Relaying sends the signal onward, amplification increases the effect of a single ligand, and integration allows the signal to be incorporated into another biochemical pathway. In this sense, a receptor is a protein-molecule that recognizes and responds to endogenous chemical signals. For example, an acetylcholine receptor recognizes and responds to its endogenous ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug targets, such as enzymes, transporters, and ion channels.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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