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Mouse Anti-AAV8 Recombinant Antibody (V2-634028) (CBMAB-AP022LY)


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Published Data
Mouse Anti-AAV8 Recombinant Antibody (ADK8) in Neutralization
Sensitivity in detecting neutralizing antibody at different MOI values

(A-D) The AAV vector (AAV5 [A and C] or AAV8 [B and D]) expressing secNanoLuc was incubated with an indicated concentration of human immunoglobulin (IVIG) (A and B) or anti-AAV monoclonal antibody (mAb) (C and D) for 1 h. CHO-K1 cells for AAV5 and Huh-7 cells for AAV8 were transduced with the AAV vector at an MOI of 100 (orange) or 1,000 (blue). Transgene expressions in the supernatant and cell lysate were determined by luminescence and expressed as transduction efficiency (%). Complete transduction (100%) was defined based on the result of the RLU obtained from incubation of the AAV vector with fetal bovine serum. Inhibition of vector transduction by neutralizing antibody is expressed as the percentage of transduction. ND50 values were calculated as the dilutions needed to neutralize 50% vector transduction. Values are presented as mean ± SD (n = 3). NTC, no transduction control. ...View More
Baatartsogt, N., Kashiwakura, Y., Hayakawa, M., Kamoshita, N., Hiramoto, T., Mizukami, H., & Ohmori, T. (2021). A sensitive and reproducible cell-based assay via secNanoLuc to detect neutralizing antibody against adeno-associated virus vector capsid. Molecular Therapy-Methods & Clinical Development, 22, 162-171.
Mouse Anti-AAV8 Recombinant Antibody (ADK8) in IF
Capsid assembly of AAV2, -4, -5, -8, and -9 in HeLa cells. HeLa cells were transiently transfected with plasmids expressing VP3 proteins derived from AAV2,-4, -5, -8, or -9 in the presence (+) or absence (−) of a cotransfected plasmid expressing their cognate AAP. The groups that received no AAP-expressing plasmid were transfected with pCMV3-GFP instead to ensure successful transfection and maintain the total quantity of transfected DNA constant across the groups. The cells were fixed at 48 h posttransfection, immunostained with the antibodies indicated in each panel, and counterstained with DAPI. The images were obtained by using a Zeiss LSM 710 confocal microscope with a 63×/1.4-NA objective. Three representative fields of view are shown for each condition. Cells were stained with anti-AAV capsid antibody (A20 for AAV2, ADK4 for AAV4, ADK5a for AAV5, ADK8 for AAV8, and ADK8/9 for AAV9) (red) and anti-FLAG antibody (white). ...View More
Earley, L. F., Powers, J. M., Adachi, K., Baumgart, J. T., Meyer, N. L., Xie, Q., ... & Nakai, H. (2017). Adeno-associated virus (AAV) assembly-activating protein is not an essential requirement for capsid assembly of AAV serotypes 4, 5, and 11. Journal of virology, 91(3), e01980-16.
Mouse Anti-AAV8 Recombinant Antibody (ADK8) in Dot blot
The cross-reactivity the anti-AAV8 antibodies against different AAV serotypes: ADK8 (Sonntag et al. 2011) as a positive control, and the new anti-AAV8 HL antibodies, HL2381 and HL2383. ...View More
Tseng, Y. S., Van Vliet, K., Rao, L., McKenna, R., Byrne, B. J., Asokan, A., & Agbandje-McKenna, M. (2016). Generation and characterization of anti-adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies. Journal of virological methods, 236, 105-110.
Mouse Anti-AAV8 Recombinant Antibody (ADK8) in ELISA
Recognition of wt rAAV2, rAAV8, and their chimeras by ADK8. (A) ADK8 ELISA for rAAV8 and rAAV8 with AAV2 amino acid substitution mutants. Wild-type rAAV8 and the mutants rAAV8456GTANTQ461→GTTTQS and rAAV8493TTTGQ497→KTSAD were detected by the ADK8, but the rAAV8586LQQQNT591→LQRGNR mutant was not. (B) ADK8 ELISA for rAAV2, rAAV8, and rAAV2 with AAV8 amino acid substitution mutants. Wild-type rAAV2 and the rAAV2457QSRLQ461→TQTLG mutant were not recognized by ADK8, but the rAAV2585RGNRQ589→QQNTA mutant was. (C) ADK8 ELISA for rAAV8 and seven-residue peptide insertion (at aa 590) mutants. All insertions disrupted AAV8 detection by ADK8. In panels A to C, the mean standard deviations from four independent experiments are shown; asterisks identify mutants for which capsids could not be detected in the ADK8 ELISA (detection limit, 108 capsids/ml). A total of 1010 viral genomes were used for each ELISA. ...View More
Gurda, B. L., Raupp, C., Popa-Wagner, R., Naumer, M., Olson, N. H., Ng, R., ... & Agbandje-McKenna, M. (2012). Mapping a neutralizing epitope onto the capsid of adeno-associated virus serotype 8. Journal of virology, 86(15), 7739-7751.

Summary

Host Animal
Mouse
Specificity
Adeno Associated Virus
Clone
V2-634028
Antibody Isotype
IgG2a, κ
Application
ELISA, WB, IP, Neutralization

Basic Information

Immunogen
Adeno-associated virus type 8 (AAV8) capsid proteins and virus particles
Host Species
Mouse
Specificity
Adeno Associated Virus
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Preservative
0.09% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Adeno-Associated Virus Serotype 8
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For research use only. Not intended for any clinical use.

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