Human Cytokine Antibody Array (Glass Slide, 640 Targets) (Y0178) (AbAr-0178-YC)

Cytokine Array

2B4 (CD244), 4-1BB Ligand (TNFSF9), 4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), ACE, ACE-2, Activin A, Activin R1B (ALK-4), Activin R2B (ACVR2B), ADAM-12, ADAM23, ADAM-8, ADAM-9, ADAMTS-13, Adiponectin (ACRP30), Adipsin (Complement Factor D), ADP-Ribosyl Cyclase 2 (BST-1/CD157), aFGF (FGF-1), Aggrecan, AgRP, Albumin, ALCAM (CD166), ALK-1, ALK-6 (BMPR-IB), Alpha-fetoprotein, AMICA, AmiGO, Aminopeptidase LRAP (ERAP2), Aminopeptidase P2 (XPNPEP2), Amnionless, Amphiregulin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, Angiotensinogen (SERPINA8), ANGPTL3, ANGPTL4, APRIL, Artemin, Arylsulfatase A (ARSA), AXL, B7-H1 (CD274), B7-H2 (ICOS Ligand), B7-H3 (CD276), BAFF, BAFF R, BAMBI (NMA), BCAM, bcl-w, BCMA, BDNF, beta IG-H3, Beta-2 Microglobulin, Betacellulin (BTC), beta-NGF, bFGF, BLAME (SLAMF8), BLC (CXCL13), BMP-2, BMP-4, BMP-5, BMP-7, BMP-8, BMP-9, BMPR-IA (ALK-3), BMPR-II, BOC (Brother of CDO), Brevican, C5a, CA12, CA125, CA15-3, CA19-9, CA9, Cadherin-11, Cadherin-13, Cadherin-4, Calcitonin, Calsyntenin-1 (Alcadein-alpha), Carboxypeptidase A2 (CPA2), Cardiotrophin-1 (CT-1), Cathepsin B (Pro-Cathepsin B), Cathepsin E, Cathepsin L, Cathepsin S, Cathepsin V (Cathepsin L2), CCL28 (MEC), CD109, CD14, CD147 (EMMPRIN), CD155 (PVR), CD163, CD200, CD229 (SLAMF3), CD23, CD26 (DPPIV), CD27 (TNFRSF7), CD28, CD30 (TNFRSF8), CD300c (CLM-6), CD320 (TCblR/8D6A), CD34, CD40 Ligand (TNFSF5), CD40 (TNFRSF5), CD48 (SLAMF2), CD58 (LFA-3), CD6, CD80 (B7-1), CD83, CD84 (SLAMF5), CD86 (B7-2), CD97, CD99, CDNF, CDO, CEA (CEACAM-5), CEA (CEACAM-5), CEACAM-1, Chemerin, CHI3L1, CHL-1 (L1CAM-2), CHST1 (GST-1), CHST4 (GST-3), cIAP-2, CILP-1, Ck beta 8-1 (CCL23/MPIF-1), CLEC-1, CLEC10A (CD301), Clusterin, CMG-2 (ANTXR2), CNTF, CNTF R alpha, Coagulation Factor VII, Coagulation Factor XIV (Protein C), Common beta Chain, Complement MASP3, Contactin-1, Contactin-2 (TAG1), CREG, CRIM1, Cripto-1, CRP (C-Reactive Protein), CRTAC1, CRTAM, CTACK (CCL27), CTLA-4 (CD152), CXADR, CXCL14 (BRAK), CXCL16, Cystatin A, Cystatin B, Cystatin C, Cystatin E/M, Cystatin SN, Cytokeratin 8 (KRT8), DAN, DcR3, Decorin, Dectin-1 (CLEC7A), Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoglein-3, DKK-1, DKK-3, DKK-4, DLL1, DNAM-1 (CD226), Dopa Decarboxylase (DDC), DPPII (QPP/DPP7), DR3 (TNFRSF25), DR6 (TNFRSF21), DSPG3, Dtk, E-Cadherin, EDA-A2, EDAR, EGF, EGFR, EG-VEGF (PK1), EMR2, ENA-78 (CXCL5), Endocan, Endoglin (CD105), Endoglycan (PODXL2), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), EphA1, EphA2, EphB4, EphB6, Ephrin-A4, Ephrin-B3, Epiregulin, ErbB2, ErbB3, ErbB4 (HER4), Erythropoietin R, ESAM, E-Selectin, FABP1, FABP2, FAP, Fas Ligand (TNFSF6), Fas (TNFRSF6/Apo-1), Fc gamma RIIB/C (CD32B/C), FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Ferritin, Fetuin A, FGF-12, FGF-17, FGF-19, FGF-20, FGF-21, FGF-23, FGF-4, FGF-5, FGF-6, FGF-7 (KGF), FGF-9, FLRG, Flt-3 Ligand, Flt-3 (Flk-2), FOLH1 (PSMA), Follistatin, Follistatin-like 1, FOLR1, FOLR2, Fractalkine (CX3CL1), FSH, Furin, Galectin-1, Galectin-2, Galectin-3, Galectin-4, Galectin-7, Galectin-8, Galectin-9, Gas 1, Gas 6, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), GCSF, GCSF R (CD114), GDF-15, GDF-8 (Myostatin), GDNF, GITR Ligand (TNFSF18), GITR (TNFRSF18), GLP-1, Glypican 1, Glypican 2, Glypican 5, GM-CSF, GM-CSF R alpha, GOLM1 (GP73), gp130 (sgp130), GPR56, GPVI, Granulysin (NKG5/LAG-2), GRO alpha/beta/gamma, GRO alpha (CXCL1), Growth Hormone, Growth Hormone R, HAI-1, HAI-2, HAPLN1, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), hCG beta, Hepsin, HGF, HGFR, HTRA2, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), ICOS, IFN-alpha/beta R2, IFN-gamma, IFN-gamma R1, IGF-1, IGF-1 R, IGF-2, IGF-2 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 R3 (IL-1 R Acp), IL-1 R4 (ST2), IL-1 R5 (IL-18 R alpha), IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 1 (IL-13 R1), IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17A, IL-17B, IL-17C, IL-17E (IL-25), IL-17F, IL-18, IL-18 BP alpha, IL-18 R beta (AcPL), IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-20, IL-20 R alpha, IL-21, IL-21 R, IL-22 R alpha 1, IL-22BP, IL-23, IL-23 R, IL-24, IL-27, IL-27 R alpha, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-3, IL-31, IL-31 RA, IL-32 alpha, IL-33 (IL-1 F11), IL-34, IL-36 alpha (IL-1 F6), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-36 Ra (IL-1 F5), IL-37 (IL-1 F7), IL-38 (IL-1 F10), IL-4, IL-4 R alpha, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha (CD127), IL-8 (CXCL8), IL-9, ILT-2 (LIR-1/CD85j), Insulin, Insulin R (CD220), Integrin alpha 5, IP-10 (CXCL10), I-TAC (CXCL11), Jagged 1, Jagged 2, JAM-A (CD321/F11R), JAM-B (VE-JAM/CD322), JAM-C (CD323), Kallikrein 14, Kallikrein 5, Kallikrein 7, KIRREL3 (NEPH2), KLF4, LAG-3, LAIR1, LAMP (LSAMP), LAMP1 (CD107a), Langerin (CD207), LAP/TGF beta 1 (TGF beta 1), Layilin, LDL R, Legumain, Leptin, Leptin R, LIF, LIF R alpha, LIGHT (TNFSF14), LIMPII, Lipocalin-1, Lipocalin-2 (NGAL), LOX-1, LRIG3, LRP-6, L-Selectin (CD62L), Lumican, Luteinizing hormone, Lymphotactin (XCL1), Lymphotoxin beta R (TNF RIII), LYVE-1, Marapsin, Matrilin-3, Matriptase (ST14), MBL, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MDGA1, MDM2, MEPE (OF45), Meprin alpha, Meprin beta (MEP1B), Mer, Mesothelin, MFRP, MICA, MICB, Midkine, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MIS RII (AMHR2), MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1 (CCL23), MSP alpha/beta, MSP Receptor (Ron), NAP-2 (PPBP/CXCL7), N-Cadherin (Cadherin-2), NCAM-1 (CD56), Nectin-1, Nectin-3, Nectin-4, Neprilysin, Neprilysin-2 (MMEL1), Neurexin-3 beta (NRXN3), Neuropilin-2, Neurturin, NGFR (TNFRSF16), Nidogen-1, Nidogen-2, NKp30, NKp44, Nogo Receptor (Reticulon-4 Receptor), Notch-1, Notch-3, NOV (CCN3), NrCAM, NRG1-beta 1 (HRG1-beta 1), NSE, NT-3, NT-4, Olfactomedin-2 (Noelin-2), Oncostatin M, Oncostatin M R beta (IL-31 R beta), Osteoactivin (GPNMB), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), OX40 Ligand (TNFSF4), OX40 (TNFRSF4), p27/Kip1 (CDKN1B), p53, PAI-1, PAPP-A (htPAPP-A), PAPP-A2, PARC (CCL18), P-Cadherin, PD-1, PD-ECGF (Thymidine Phosphorylase), PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PECAM-1, Pentraxin-3 (TSG-14), Pepsinogen 1, Pepsinogen 2, Periostin, Persephin, PGRPs, Platelet Factor 4 (CXCL4), Plexin B3, Plexin D1, PLGF, Pref-1 (DLK-1/FA1), Presenilin 1, Procalcitonin, Progranulin, ProGRP, Prolactin, Prolactin R, Prostasin, PSA-free, PSA-total, PTH, PYY, RAGE, RANK (TNFRSF11A), RANTES (CCL5), RBP4, Reg1B, RELT (TNFRSF19L), Renin, Resistin, Ret, RGM-A, RGM-B, ROBO2, ROBO3, ROBO4, Ryk, S100 A8, SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), Semaphorin 6B, Semaphorin 6D, Semaphorin 7A, SERPINA4, SERPINF1 (PEDF), sFRP-3, SIGIRR, Siglec-10, Siglec-11, Siglec-5 (CD170), Siglec-7 (CD328), Siglec-9, SLAM (CD150), Sonic Hedgehog N-Terminal (Shh-N), SOST, SOX2, SP-D, Spinesin, SREC-I (SCARF1), SREC-II (SCARF2), Syndecan-1, Syndecan-3, Syndecan-4, TACE, TACI (TNFRSF13B), TARC (CCL17), TECK (CCL25), Testican 2 (SPOCK2), TFF3 (Trefoil Factor 3), TFPI, TFPI-2, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, TGF beta R3, Thrombomodulin, Thrombopoietin (TPO), Thrombospondin 1, Thrombospondin 2, Thrombospondin 5 (COMP), Thyroglobulin, Tie-1, Tie-2, TIM-1 (KIM-1), TIM-3 (KIM-3), TIMP-1, TIMP-2, TIMP-4, TLR1, TLR2, TLR3, TLR4, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRACP (PAP/ACP5), TRAIL R1 (TNFRSF10A/DR4), TRAIL R2 (TNFRSF10B/DR5), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRAIL (TNFSF10), TRANCE (TNFSF11), Transferrin, Transferrin, Trappin-2, TREM-1, TREM-2, Tripeptidyl-peptidase I (TPP1), TRKC, TROP1 (EpCAM), Troponin I, TROY (TNFRSF19), TSH, TSLP, TWEAK R (TNFRSF12), TWEAK (TNFSF12), Ubiquitin+1, ULBP-1, ULBP-2 (ALCAN-alpha/N2DL2), ULBP-3, uPA, uPAR, Uromodulin, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, WIF-1, WISP-1 (CCN4), XEDAR, XIAP (BIRC4/IAP-3)

Human

Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Other Body Fluids

Multiplex Protein Detection

Quantitative

Glass Slide

Sandwich-based

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Cytokines are a broad and loose category of small proteins (5–20 kDa) that are important in cell signaling. Cytokines are peptides, and cannot cross the lipid bilayer of cells to enter the cytoplasm. Cytokines have been shown to be involved in autocrine signaling, paracrine signaling and endocrine signaling as immunomodulating agents. Their definite distinction from hormones is still part of ongoing research. Cytokines include chemokines, interferons, interleukins, lymphokines, and tumour necrosis factors, but generally not hormones or growth factors (despite some overlap in the terminology). Cytokines are produced by a broad range of cells, including immune cells like macrophages, B lymphocytes, T lymphocytes and mast cells, as well as endothelial cells, fibroblasts, and various stromal cells; a given cytokine may be produced by more than one type of cell.