Rat Cytokine Antibody Array (Membrane, 34 Targets) (Y0091) (AbAr-0091-YC)

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Datasheet

Specifications

Target Array

Cytokine Array

Target

Activin A, CINC-2, GM-CSF, IL-1 R6 (IL-1 Rrp2), IL-13, MIP-3 alpha (CCL20), TCK-1 (CXCL7), Agrin, CINC-3, ICAM-1 (CD54), IL-2, Leptin, MMP-8, TIMP-1, CD86 (B7-2), CNTF, IFN-gamma, IL-4, LIX, PDGF-AA, TNF alpha, beta-NGF, Fas Ligand (TNFSF6), IL-1 alpha (IL-1 F1), IL-6, L-Selectin (CD62L), Prolactin R, VEGF-A, CINC-1 (CXCL1), Fractalkine (CX3CL1), IL-1 beta (IL-1 F2), IL-10, MCP-1 (CCL2), RAGE

Species Reactivity

Rat

Sample Type

Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application

Multiplex Protein Detection

Assay Type

Semi-Quantitative

Solid Support

Membrane

Detection Assay

Sandwich-based

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Components

Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction

Cytokines are a broad and loose category of small proteins (5–20 kDa) that are important in cell signaling. Cytokines are peptides, and cannot cross the lipid bilayer of cells to enter the cytoplasm. Cytokines have been shown to be involved in autocrine signaling, paracrine signaling and endocrine signaling as immunomodulating agents. Their definite distinction from hormones is still part of ongoing research. Cytokines include chemokines, interferons, interleukins, lymphokines, and tumour necrosis factors, but generally not hormones or growth factors (despite some overlap in the terminology). Cytokines are produced by a broad range of cells, including immune cells like macrophages, B lymphocytes, T lymphocytes and mast cells, as well as endothelial cells, fibroblasts, and various stromal cells; a given cytokine may be produced by more than one type of cell.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Human Cytokine Antibody Array (Glass Slide, 60 Targets) (Y0049)

Human Cytokine Antibody Array (Glass Slide, 80 Targets) (Y0037)

Human Cytokine Antibody Array (Glass Slide, 274 Targets) (Y0036)

Human Cytokine Antibody Array (Glass Slide, 42 Targets) (Y0035)

Human Cytokine Antibody Array (Glass Slide, 174 Targets) (Y0034)

Human Cytokine Antibody Array (Glass Slide, 49 Targets) (Y0033)

Human Cytokine Antibody Array (Glass Slide, 120 Targets) (Y0032)

Mouse Cytokine Antibody Array (Glass Slide, 144 Targets) (Y0069)

Mouse Cytokine Antibody Array (Membrane, 62 Targets) (Y0063)

Mouse Cytokine Antibody Array (Membrane, 32 Targets) (Y0062)

Antibody Array

Human Cytokine Antibody Array (Membrane, 120 Targets) (Y0020)

Human Cytokine Antibody Array (Membrane, 49 Targets) (Y0021)

Canine Cytokine Antibody Array (Membrane, 40 Targets) (Y0022)

Human Cytokine Antibody Array (Membrane, 174 Targets) (Y0023)

Human Cytokine Antibody Array (Membrane, 42 Targets) (Y0024)

Human Cytokine Antibody Array (Membrane, 274 Targets) (Y0025)

Human Cytokine Antibody Array (Membrane, 36 Targets) (Y0026)

Human Cytokine Antibody Array (Membrane, 80 Targets) (Y0027)

Human Cytokine Antibody Array (Membrane, 60 Targets) (Y0028)

Human Cytokine Antibody Array (Membrane, 60 Targets) (Y0029)

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Porcine Cytokine Antibody Array (Glass Slide, 10 Targets) (Y0138)

Mouse Cytokine Antibody Array (Glass Slide, 62 Targets) (Y0071)

Rat Cytokine Antibody Array (Glass Slide, 27 Targets) (Y0124)

Mouse Cytokine Antibody Array (Glass Slide, 40 Targets) (Y0417)

Human Cytokine Antibody Array (Glass Slide, 40 Targets) (Y0466)

Porcine Cytokine Antibody Array (Glass Slide, 50 Targets) (Y0435)

Mouse Cytokine Antibody Array (Glass Slide, 80 Targets) (Y0460)

Human Cytokine Antibody Array (Glass Slide, 40 Targets) (Y0095)

Human Cytokine Antibody Array (Membrane, 36 Targets) (Y0026)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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