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Human Autoimmune Disease IgG Autoantibody Array (Glass Slide, 33 Targets) (Y0216) (AbAr-0216-YC)


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Specifications

Target Array
Autoimmune Disease IgG Autoantibody Array
Target
Alanyl-tRNA Synthetase, Gliadin, KRT19, M/Scl 100, Calcitonin, DNA topoisomerase 1, Thrombopoietin, KRT8, Glycine-tRNA ligase (AP-4-A synthetase/GlyRS), Histidine-tRNA ligase (HisRS), M2, PCNA, Sm core protein, TGM2, CENP-B, POM210, Lupus La protein (SS-B), Mi-2, Myeloblastin (PR3/C-ANCA), Sp-100, snRNP70, CENP-A, Histone H2A, LC-1, Myeloperoxidase, TRIM21, Threonine-tRNA ligase (ThrRS), dsDNA, Histone H2B, KRT20, Nucleoporin Nup62, TRIM21, Thyroglobulin
Species Reactivity
Human
Sample Type
Serum

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Thoroughly dry the glass slide and block the array surface to prevent non-specific binding.
Incubate the array with both samples and standards.
Incubate with a biotinylated detection antibody cocktail and then with streptavidin-conjugated fluorescent dye.
Carefully disassemble the glass slide and scan the slide using a gene microarray laser scanner.
Conduct densitometric analysis.
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
An autoimmune disease is a condition arising from an abnormal immune response to a normal body part. There are at least 80 types of autoimmune diseases. Nearly any body part can be involved. Common symptoms include low grade fever and feeling tired.
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For research use only. Not intended for any clinical use.

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