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Mouse Anti-VRK1 Recombinant Antibody (CBXS-0943) (CBMAB-S3857-CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXS-0943
Application
WB, ICC, IHC-P, IHC-Fr, ELISA

Basic Information

Immunogen
Vaccinia Related Kinase 1
Specificity
Human
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 50% glycerol
Preservative
0.02% sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Vaccinia Related Kinase 1
Introduction
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.
Entrez Gene ID
7443
UniProt ID
Q99986
Alternative Names
Vaccinia Related Kinase 1; EC 2.7.11.1; Serine/Threonine-Protein Kinase VRK1; Vaccinia Virus B1R-Related Kinase 1; Vaccinia-Related Kinase 1; PCH1A; PCH1;
Function
Serine/threonine kinase involved in cell cycle, nuclear condensation and transcription regulation (PubMed:14645249, PubMed:18617507, PubMed:19103756).
Involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation (PubMed:19103756).
Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2 (PubMed:10951572).
Phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity (PubMed:33076429).
Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (PubMed:16495336).
Phosphorylates ATF2 which activates its transcriptional activity (PubMed:15105425).
Biological Process
Biological Process cell division Source:UniProtKB-KW
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process Golgi disassembly Source:UniProtKB1 Publication
Biological Process mitotic nuclear membrane disassembly Source:Reactome
Biological Process peptidyl-serine phosphorylation Source:GO_Central1 Publication
Biological Process positive regulation of protein localization to chromatin Source:UniProtKB1 Publication
Biological Process protein autophosphorylation Source:UniProtKB2 Publications
Biological Process protein phosphorylation Source:ProtInc1 Publication
Biological Process signal transduction Source:GO_Central1 Publication
Cellular Location
Nucleus
Cytoplasm
Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
Involvement in disease
Pontocerebellar hypoplasia 1A (PCH1A):
A disorder characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and spinal cord anterior horn cells degeneration resembling infantile spinal muscular atrophy. Additional features include muscle hypotonia, congenital contractures and respiratory insufficiency that is evident at birth.
PTM
Autophosphorylated at various serine and threonine residues (PubMed:11883897, PubMed:14645249, PubMed:19103756, PubMed:21543316).
Autophosphorylation does not impair its ability to phosphorylate p53/TP53 (PubMed:11883897).
Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis (PubMed:19103756).
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For research use only. Not intended for any clinical use.

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