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Mouse Anti-VASP Recombinant Antibody (1F3) (CBMAB-1294-YY)


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Summary

Host Animal
Mouse
Specificity
Human, Dog
Clone
1F3
Antibody Isotype
IgG1
Application
WB, IHC

Basic Information

Immunogen
Full length human recombinant protein of human VASP (NP_003361) produced in HEK293T cell
Specificity
Human, Dog
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Vasodilator Stimulated Phosphoprotein
Entrez Gene ID
Human7408
Dog403936
UniProt ID
HumanP50552
DogP50551
Function
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Biological Process
Actin cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Actin polymerization or depolymerizationIEA:InterPro
Axon guidanceManual Assertion Based On ExperimentIBA:GO_Central
Neural tube closureManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of actin filament polymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Protein homotetramerizationIEA:InterPro
Cellular Location
Cytoplasm
Cytoplasm, cytoskeleton
Cell junction, focal adhesion
Cell junction, tight junction
Cell projection, lamellipodium membrane
Cell projection, filopodium membrane
Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes).
PTM
Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG/PRKG1, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation at Ser-157 by PKA is required for localization to the tight junctions in epithelial cells. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Phosphorylation at Ser-322 by AMPK also alters actin filament binding. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.
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For research use only. Not intended for any clinical use.

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