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Mouse Anti-TUBB3 Recombinant Antibody (ONS.1A6) (CBMAB-1848-CN)


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Summary

Host Animal
Mouse
Specificity
Human, Cattle, Hamster, Mouse, Rat
Clone
ONS.1A6
Antibody Isotype
IgG1
Application
WB, IHC, ELISA

Basic Information

Immunogen
Synthetic peptide conjugated to BSA, corresponding to the C-terminal sequence of beta4 tubulin.
Specificity
Human, Cattle, Hamster, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Ascites
Preservative
15mM Sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
TUBB3 Gene(Protein Coding) Tubulin Beta 3 Class III
Introduction
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Entrez Gene ID
Human10381
Mouse22152
Rat246118
Hamster101831134
Cattle768070
UniProt ID
HumanQ13509
MouseQ9ERD7
RatQ4QRB4
HamsterA0A1U7QRM6
CattleQ2T9S0
Alternative Names
CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871).
Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871).
Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871).
TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521).
Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977).
Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).
Biological Process
Biological Process axon guidance Source:UniProtKB1 Publication
Biological Process dorsal root ganglion development Source:UniProtKB1 Publication
Biological Process microtubule cytoskeleton organization Source:UniProtKB1 Publication
Biological Process mitotic cell cycle Source:GO_Central1 Publication
Cellular Location
Cytoplasm, cytoskeleton
Cell projection, growth cone
Cell projection, lamellipodium
Cell projection, filopodium
Involvement in disease
Fibrosis of extraocular muscles, congenital, 3A (CFEOM3A):
A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases, the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
Cortical dysplasia, complex, with other brain malformations 1 (CDCBM1):
A disorder of aberrant neuronal migration and disturbed axonal guidance. Affected individuals have mild to severe intellectual disability, strabismus, axial hypotonia, and spasticity. Brain imaging shows variable malformations of cortical development, including polymicrogyria, gyral disorganization, and fusion of the basal ganglia, as well as thin corpus callosum, hypoplastic brainstem, and dysplastic cerebellar vermis. Extraocular muscles are not involved.
PTM
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866).
Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Glutamylation is also involved in cilia motility (By similarity).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
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For research use only. Not intended for any clinical use.

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