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Mouse Anti-TUBB Recombinant Antibody (CB138A) (CBMAB-CA138LY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CB138A
Antibody Isotype
IgG
Application
ELISA, WB

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
TUBB Gene(Protein Coding) Tubulin Beta Class I
Introduction
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
Entrez Gene ID
203068
UniProt ID
P07437
Alternative Names
Tubulin Beta Class I; Tubulin, Beta Polypeptide; Tubulin Beta-5 Chain; TUBB5; Tubulin, Beta Class I; Class I Beta-Tubulin; Tubulin Beta-1 Chain; Tubulin Beta Chain; Beta Ib Tubulin;
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Biological Process
Biological Process cell division Source:BHF-UCL1 Publication
Biological Process cytoskeleton-dependent intracellular transport Source:BHF-UCL1 Publication
Biological Process microtubule cytoskeleton organization Source:GO_Central1 Publication
Biological Process microtubule-based process Source:BHF-UCL1 Publication
Biological Process mitotic cell cycle Source:GO_Central1 Publication
Biological Process natural killer cell mediated cytotoxicity Source:UniProtKB1 Publication
Biological Process odontoblast differentiation Source:GO_Central1 Publication
Biological Process regulation of synapse organization Source:Ensembl
Biological Process spindle assembly Source:Ensembl
Cellular Location
Cytoplasm, cytoskeleton
Involvement in disease
Cortical dysplasia, complex, with other brain malformations 6 (CDCBM6):
A disorder of aberrant neuronal migration and disturbed axonal guidance. Affected individuals have microcephaly, ataxia, and severe delayed psychomotor development. Brain imaging shows variable malformations of cortical development, including white matter streaks, dysmorphic basal ganglia, corpus callosum abnormalities, brainstem and cerebellar hypoplasia, cortical dysplasia, polymicrogyria.
Skin creases, congenital symmetric circumferential, 1 (CSCSC1):
An autosomal dominant disease characterized by multiple, symmetric, circumferential rings of folded skin, affecting primarily the limbs. Affected individuals also exhibit intellectual disability, cleft palate, and dysmorphic features.
PTM
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866, PubMed:28576883).
Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Glutamylation is also involved in cilia motility (By similarity).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility (Probable) (PubMed:28576883).
Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally (Probable) (PubMed:28576883).
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
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For research use only. Not intended for any clinical use.

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