Hamster Anti-Traf6 Recombinant Antibody (CBYJT-4530) (CBMAB-T4036-YJ)

Hamster

Mouse

CBYJT-4530

IgG

IP, WB

TRAF6 antibody was raised against recombinant Mouse TRAF6.

Mouse

IgG

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

PBS, pH 7.2, 50% Glycerol

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

TRAF6 Gene(Protein Coding) TNF Receptor Associated Factor 6

Traf6 is a member of the TNF receptor associated factor (TRAF) family of adaptor proteins that mediate signaling events from members of the TNF receptor and Toll/IL-1 receptor families to activate transcription factors such as NF-kappa-B and AP-1. Traf6 is essential for perinatal and postnatal survival. Mice deficient in Traf6 exhibit osteopetrosis and defective in development of epidermal appendixes, normal B cell differentiation, lymph node organogenesis, interleukin-1 signaling, lipopolysaccharide signaling and neural tube closure. Traf6 possesses ubiquitin ligase activity.

AI851288; 2310003F17Rik; C630032O20Rik

E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2 (PubMed:11057907, PubMed:18347055, PubMed:19713527, PubMed:19465916).
Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation (PubMed:19675569).
Leads to the activation of NF-kappa-B and JUN (PubMed:16378096, PubMed:17135271).
Seems to also play a role in dendritic cells (DCs) maturation and/or activation (By similarity).
Represses c-Myb-mediated transactivation, in B-lymphocytes (PubMed:18093978, PubMed:18758450).
Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor (PubMed:8837778, PubMed:19825828, PubMed:12140561).
Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation (By similarity).
Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production (By similarity).
Participates also in the TCR signaling by ubiquitinating LAT (PubMed:25907557, PubMed:23514740).

Biological Process activation of NF-kappaB-inducing kinase activity Source:UniProtKB1 Publication
Biological Process activation of protein kinase activity Source:UniProtKB1 Publication
Biological Process antigen processing and presentation of exogenous peptide antigen via MHC class II Source:Ensembl
Biological Process bone resorption Source:Ensembl
Biological Process cell development Source:Ensembl
Biological Process cellular response to cytokine stimulus Source:ARUK-UCL1 Publication
Biological Process cellular response to DNA damage stimulus Source:UniProtKB-KW
Biological Process cellular response to lipopolysaccharide Source:MGI1 Publication
Biological Process cytoplasmic pattern recognition receptor signaling pathway Source:Reactome
Biological Process Fc-epsilon receptor signaling pathway Source:Reactome
Biological Process I-kappaB kinase/NF-kappaB signaling Source:Reactome
Biological Process in utero embryonic development Source:Ensembl
Biological Process interleukin-1-mediated signaling pathway Source:GO_Central1 Publication
Biological Process interleukin-17-mediated signaling pathway Source:Ensembl
Biological Process MyD88-dependent toll-like receptor signaling pathway Source:Reactome
Biological Process myeloid dendritic cell differentiation Source:Ensembl
Biological Process negative regulation of DNA-templated transcription Source:UniProtKB1 Publication
Biological Process negative regulation of transcription by RNA polymerase II Source:UniProtKB1 Publication
Biological Process neural tube closure Source:Ensembl
Biological Process odontogenesis of dentin-containing tooth Source:Ensembl
Biological Process ossification Source:UniProtKB-KW
Biological Process osteoclast differentiation Source:Ensembl
Biological Process positive regulation of DNA-binding transcription factor activity Source:BHF-UCL1 Publication
Biological Process positive regulation of I-kappaB kinase/NF-kappaB signaling Source:UniProtKB1 Publication
Biological Process positive regulation of interleukin-12 production Source:Ensembl
Biological Process positive regulation of interleukin-2 production Source:UniProtKB1 Publication
Biological Process positive regulation of interleukin-6 production Source:Ensembl
Biological Process positive regulation of JNK cascade Source:GO_Central1 Publication
Biological Process positive regulation of JUN kinase activity Source:BHF-UCL1 Publication
Biological Process positive regulation of leukocyte adhesion to vascular endothelial cell Source:ARUK-UCL1 Publication
Biological Process positive regulation of lipopolysaccharide-mediated signaling pathway Source:Ensembl
Biological Process positive regulation of NF-kappaB transcription factor activity Source:UniProtKB2 Publications
Biological Process positive regulation of NIK/NF-kappaB signaling Source:ARUK-UCL1 Publication
Biological Process positive regulation of osteoclast differentiation Source:UniProtKB1 Publication
Biological Process positive regulation of protein ubiquitination Source:BHF-UCL1 Publication
Biological Process positive regulation of T cell cytokine production Source:UniProtKB1 Publication
Biological Process positive regulation of T cell proliferation Source:Ensembl
Biological Process positive regulation of transcription by RNA polymerase II Source:BHF-UCL1 Publication
Biological Process positive regulation of transcription regulatory region DNA binding Source:BHF-UCL1 Publication
Biological Process protein autoubiquitination Source:UniProtKB2 Publications
Biological Process protein K63-linked ubiquitination Source:UniProtKB2 Publications
Biological Process protein polyubiquitination Source:UniProtKB2 Publications
Biological Process regulation of apoptotic process Source:InterPro
Biological Process regulation of I-kappaB kinase/NF-kappaB signaling Source:GO_Central1 Publication
Biological Process regulation of immunoglobulin production Source:Ensembl
Biological Process response to interleukin-1 Source:UniProtKB1 Publication
Biological Process stimulatory C-type lectin receptor signaling pathway Source:Reactome
Biological Process T cell receptor signaling pathway Source:UniProtKB1 Publication
Biological Process T-helper 1 type immune response Source:Ensembl
Biological Process tumor necrosis factor-mediated signaling pathway Source:GO_Central1 Publication

Cytoplasm
Cytoplasm, cell cortex
Nucleus
Lipid droplet
Found in the nuclei of some aggressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T- and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus. RSAD2/viperin recruits it to the lipid droplet (By similarity).

Sumoylated on Lys-124, Lys-142 and Lys-453 with SUMO1.
Polyubiquitinated on Lys-124 by TRAF3IP2; after cell stimulation with IL17A (PubMed:19825828).
Polyubiquitinated on Lys-124; after cell stimulation with IL1B or TGFB. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form. Deubiquitinated by USP10 in a TANK-dependent manner, leading to the negative regulation of NF-kappaB signaling upon DNA damage (PubMed:25861989).
LRRC19 induces 'Lys-63' ubiquitination (PubMed:25026888).