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Mouse Anti-TP53BP1 Recombinant Antibody (6B3E10) (CBMAB-1922CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
6B3E10
Antibody Isotype
IgG1
Application
ELISA, FC

Basic Information

Immunogen
The immunogen corresponding to a region within amino acids 574 and 773 of human TP53BP1 expressed in E. Coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
tumor protein p53 binding protein 1
Introduction
This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. Diseases associated with TP53BP1 include Myeloproliferative Disorder, Chronic, With Eosinophilia and Riddle Syndrome. Among its related pathways are SUMOylation and Metabolism of proteins.
Entrez Gene ID
7158
UniProt ID
Q12888
Alternative Names
TP53; p202; 53BP1; TDRD30; p53BP1
Function
Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:27153538, PubMed:28241136).
Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306, PubMed:27153538).
In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136).
Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:27153538, PubMed:28241136, PubMed:17190600).
Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425).
Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity).
In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity).
Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).
Biological Process
Biological Process cellular response to DNA damage stimulus Source:UniProtKB2 Publications
Biological Process cellular response to X-ray Source:Ensembl
Biological Process DNA damage checkpoint signaling Source:GO_Central1 Publication
Biological Process double-strand break repair via nonhomologous end joining Source:UniProtKB4 Publications
Biological Process negative regulation of double-strand break repair via homologous recombination Source:UniProtKB2 Publications
Biological Process positive regulation of DNA-binding transcription factor activity Source:BHF-UCL1 Publication
Biological Process positive regulation of DNA-templated transcription Source:UniProtKB1 Publication
Biological Process positive regulation of isotype switching Source:UniProtKB1 Publication
Biological Process positive regulation of transcription by RNA polymerase II Source:BHF-UCL1 Publication
Biological Process protein homooligomerization Source:UniProtKB1 Publication
Cellular Location
Nucleus
Chromosome
Chromosome, centromere, kinetochore
Localizes to the nucleus in absence of DNA damage (PubMed:28241136).
Following DNA damage, recruited to sites of DNA damage, such as double stand breaks (DSBs): recognizes and binds histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23333306, PubMed:23760478, PubMed:24703952, PubMed:28241136, PubMed:17190600).
Associated with kinetochores during mitosis (By similarity).
Involvement in disease
A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
PTM
Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage (PubMed:11042216, PubMed:11331310).
Phosphorylated by ATM in response to DNA damage: phosphorylation at different sites promotes interaction with different set of proteins: phosphorylation at the N-terminus by ATM (residues from 6-178) promotes interaction with PAXIP1 and non-homologous end joining (NHEJ) of dysfunctional telomeres (PubMed:23727112).
Phosphorylation by ATM at residues that are located more C-terminus (residues 300-650) leads to promote interaction with RIF1 (PubMed:23727112, PubMed:23333306, PubMed:28241136).
Interaction with RIF1 leads to disrupt interaction with NUDT16L1/TIRR (PubMed:28241136).
Phosphorylation at Thr-1609 and Ser-1618 in the UDR motif blocks interaction with H2AK15ub (PubMed:24703952).
Dephosphorylated by PPP4C (PubMed:24703952).
Hyperphosphorylation during mitosis correlates with its exclusion from chromatin and DNA lesions. Hyperphosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation (PubMed:17553757, PubMed:21144835).
Dephosphorylated by PPP5C (PubMed:19176521).
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For research use only. Not intended for any clinical use.

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