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Rat Anti-Ticam2 Recombinant Antibody (CBYJT-3128) (CBMAB-T2396-YJ)


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Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBYJT-3128
Antibody Isotype
IgG2b
Application
ICC

Basic Information

Immunogen
E. coli-derived recombinant mouse TRAM/TICAM2, Met1-Ala232, Accession # Q8BJQ4
Specificity
Mouse
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, Trehalose
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-232

Target

Full Name
TICAM2 Gene(Protein Coding) Toll Like Receptor Adaptor Molecule 2
Introduction
Ticam2 plays a role as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Ticam2 physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1; signaling is proposed to occur in early endosomes after endocytosis of TLR4. Ticam2 may also be involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB; however, reports are controversial. It is involved in IL-18 signaling and is proposed to play a role as a sorting adaptor for MYD88 in IL-18 signaling during adaptive immune response.
Entrez Gene ID
225471
UniProt ID
Q8BJQ4
Alternative Names
TRAM; Tirp; Trif; TICAM-2; B430113A10
Function
Functions as sorting adapter in different signaling pathways to facilitate downstream signaling leading to type I interferon induction (PubMed:16603631, PubMed:16757566, PubMed:25385819, PubMed:25825441).
In TLR4 signaling, physically bridges TLR4 and TICAM1 and functionally transmits signal to TICAM1 in early endosomes after endocytosis of TLR4. In TLR2 signaling, physically bridges TLR2 and MYD88 and is required for the TLR2-dependent movement of MYD88 to endosomes following ligand engagement (PubMed:25385819).
Involved in IL-18 signaling and is proposed to function as a sorting adapter for MYD88 in IL-18 signaling during adaptive immune response (PubMed:22685567).
Forms a complex with RAB11FIP2 that is recruited to the phagosomes to promote the activation of the actin-regulatory GTPases RAC1 and CDC42 and subsequent phagocytosis of Gram-negative bacteria (PubMed:30883606).
Isoform 2
Proposed to inhibit LPS-TLR4 signaling at the late endosome by interaction with isoform 1 thereby disrupting the association of isoform 1 with TICAM1. May be involved in TLR4 degradation in late endosomes.
Biological Process
Biological Process cellular response to lipopolysaccharideSource:UniProtKB1 Publication
Biological Process endoplasmic reticulum to Golgi vesicle-mediated transportSource:GO_Central1 Publication
Biological Process Golgi organizationSource:GO_Central1 Publication
Biological Process inflammatory responseSource:UniProtKB-KW
Biological Process innate immune responseSource:UniProtKB-KW
Biological Process intracellular protein transportSource:GO_Central1 Publication
Biological Process negative regulation of chemokine (C-C motif) ligand 5 productionSource:UniProtKB
Biological Process negative regulation of toll-like receptor 4 signaling pathwaySource:UniProtKB
Biological Process phagocytosisSource:UniProtKB1 Publication
Biological Process positive regulation of chemokine (C-C motif) ligand 5 productionSource:UniProtKB
Biological Process positive regulation of I-kappaB kinase/NF-kappaB signalingSource:UniProtKB1 Publication
Biological Process positive regulation of interleukin-18-mediated signaling pathwaySource:UniProtKB1 Publication
Biological Process positive regulation of toll-like receptor 4 signaling pathwaySource:UniProtKB1 Publication
Biological Process TRAM-dependent toll-like receptor 4 signaling pathwaySource:UniProtKB1 Publication
Cellular Location
Isoform 1
Cytoplasm
Golgi apparatus
Cell membrane
Endoplasmic reticulum
Early endosome membrane
Late endosome membrane
Cell projection, phagocytic cup
Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane. Upon LPS stimulation colcoalizes with isoform 2 in late endosomes.
Isoform 2
Endoplasmic reticulum
Early endosome membrane
Late endosome membrane
Translocates to late endosomes upon LPS stimulation where it colcoalizes with isoform 1.
PTM
Phosphorylated by PRKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation. Tyrosine phosphorylation is inhibited by phosphatase PTPN4.
Isoform 1 is myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling.
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For research use only. Not intended for any clinical use.

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