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Mouse Anti-TFRC Recombinant Antibody (MEM-189) (CBMAB-C9373-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
MEM-189
Antibody Isotype
IgG1
Application
FC, IP, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Preservative
15 mM sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
TFRC Gene(Protein Coding) Transferrin Receptor
Introduction
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Entrez Gene ID
7037
UniProt ID
P02786
Alternative Names
Transferrin Receptor; TFR1; TRFR; P90; TFR; TR; T9;
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738).
Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity).
A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738).
When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738).
When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Biological Process
Biological Process acute-phase responseSource:Ensembl
Biological Process agingSource:Ensembl
Biological Process cellular iron ion homeostasisSource:GO_Central1 Publication
Biological Process cellular response to leukemia inhibitory factorSource:Ensembl
Biological Process cellular response to xenobiotic stimulusSource:MGI1 Publication
Biological Process intracellular signal transductionSource:UniProtKB1 Publication
Biological Process iron ion transportSource:UniProtKB1 Publication
Biological Process negative regulation of apoptotic processSource:ARUK-UCL1 Publication
Biological Process negative regulation of mitochondrial fusionSource:UniProtKB1 Publication
Biological Process osteoclast differentiationSource:Ensembl
Biological Process positive regulation of B cell proliferationSource:UniProtKB1 Publication
Biological Process positive regulation of bone resorptionSource:Ensembl
Biological Process positive regulation of gene expressionSource:ARUK-UCL1 Publication
Biological Process positive regulation of I-kappaB kinase/NF-kappaB signalingSource:ARUK-UCL1 Publication
Biological Process positive regulation of isotype switchingSource:UniProtKB1 Publication
Biological Process positive regulation of NF-kappaB transcription factor activitySource:ARUK-UCL1 Publication
Biological Process positive regulation of peptidyl-serine phosphorylationSource:ARUK-UCL1 Publication
Biological Process positive regulation of protein localization to nucleusSource:ARUK-UCL1 Publication
Biological Process positive regulation of protein phosphorylationSource:ARUK-UCL1 Publication
Biological Process positive regulation of protein-containing complex assemblySource:ARUK-UCL1 Publication
Biological Process positive regulation of T cell proliferationSource:UniProtKB1 Publication
Biological Process receptor internalizationSource:UniProtKB1 Publication
Biological Process response to copper ionSource:Ensembl
Biological Process response to hypoxiaSource:Ensembl
Biological Process response to iron ionSource:Ensembl
Biological Process response to manganese ionSource:Ensembl
Biological Process response to nutrientSource:Ensembl
Biological Process response to retinoic acidSource:Ensembl
Biological Process transferrin transportSource:UniProtKB1 Publication
Biological Process transport across blood-brain barrierSource:ARUK-UCL2 Publications
Cellular Location
Cell membrane
Melanosome
Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Transferrin receptor protein 1, serum form
Secreted
Involvement in disease
Immunodeficiency 46 (IMD46):
An autosomal recessive primary immunodeficiency disorder characterized by early-onset chronic diarrhea, recurrent infections, hypo- or agammaglobulinemia, normal lymphocyte counts, intermittent neutropenia, and intermittent thrombocytopenia.
Topology
Cytoplasmic: 1-67
Helical: 68-88
Extracellular: 89-760
PTM
Stearoylated by ZDHHC6 which inhibits TFRC-mediated activation of the JNK pathway and promotes mitochondrial fragmentation (PubMed:26214738).
Stearoylation does not affect iron uptake (PubMed:26214738).
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
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For research use only. Not intended for any clinical use.

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