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Mouse Anti-Tbp Recombinant Antibody (CBYJT-2118) (CBMAB-T1220-YJ)


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Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Human
Clone
CBYJT-2118
Antibody Isotype
IgG
Application
WB, IP, ELISA

Basic Information

Immunogen
recombinant TFIID of mouse origin
Specificity
Mouse, Rat, Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
TBP TATA-Box Binding Protein
Introduction
Tbp is a general transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Entrez Gene ID
Human6908
Mouse21374
Rat117526
UniProt ID
HumanP20226
MouseP29037
RatQ66HB1
Alternative Names
GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID
Function
The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473).
TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473, PubMed:27193682, PubMed:2194289, PubMed:2363050, PubMed:2374612).
The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473, PubMed:27007846).
The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed:33795473).
TBP forms the TFIID-A module together with TAF3 and TAF5 (PubMed:33795473).
TBP is a general transcription factor that functions at the core of the TFIID complex (PubMed:33795473, PubMed:27193682, PubMed:2194289, PubMed:2363050, PubMed:2374612, PubMed:9836642).
During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box (PubMed:33795473).
Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071).
Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription (PubMed:15970593).
The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter (PubMed:15970593).
SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA (PubMed:15970593).
Biological Process
Biological Process DNA-templated transcription initiationIBA:GO_Central1 Publication
Biological Process mRNA transcription by RNA polymerase IIIDA:ComplexPortal1 Publication
Biological Process positive regulation of transcription initiation by RNA polymerase IIIDA:ComplexPortal1 Publication
Biological Process protein phosphorylationIDA:ComplexPortal1 Publication
Biological Process RNA polymerase II preinitiation complex assemblyIDA:CAFA1 Publication
Biological Process transcription by RNA polymerase IIIDA:BHF-UCL1 Publication
Biological Process transcription by RNA polymerase IIIIDA:MGI1 Publication
Biological Process transcription initiation at RNA polymerase II promoterTAS:ProtInc1 Publication
Cellular Location
Nucleus
Involvement in disease
Spinocerebellar ataxia 17 (SCA17):
Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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