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Mouse Anti-SPARC Antibody (AON-1) (CBMAB-1615CQ)


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Summary

Host Animal
Mouse
Specificity
Cattle, Dog, Human, Monkey, Pig, Rabbit, Rat
Clone
AON-1
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IHC, IP, WB

Basic Information

Immunogen
Osteonectin Purified from bone
Specificity
Cattle, Dog, Human, Monkey, Pig, Rabbit, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
SPARC
Introduction
This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Diseases associated with SPARC include Osteogenesis Imperfecta, Type Xvii and Sparc-Related Osteogenesis Imperfecta. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and ECM proteoglycans.
Entrez Gene ID
Human6678
Cattle282077
Rat24791
Monkey712388
Dog100855736
Pig595124
Rabbit100009355
UniProt ID
HumanP09486
CattleP13213
RatP16975
MonkeyG7MVP0
DogA0A8C0QBH2
PigP20112
RabbitP36233
Alternative Names
secreted protein acidic and cysteine rich
Function
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
Biological Process
Biological Process anatomical structure developmentManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of angiogenesisManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of endothelial cell proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of endothelial cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of cell morphogenesisManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Secreted, extracellular space, extracellular matrix, basement membrane
In or around the basement membrane.
Involvement in disease
Osteogenesis imperfecta 17 (OI17):
An autosomal recessive form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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