Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Rabbit Anti-QTRT1 Recombinant Antibody (RB50086) (CBMAB-01937-YC)


See all QTRT1 antibodies
Online Inquiry

Summary

Host Animal
Rabbit
Specificity
Human
Clone
RB50086
Antibody Isotype
IgG
Application
ELISA, WB

Basic Information

Immunogen
A KLH conjugated synthetic peptide between 303-337 amino acids from the C-terminal region of human QTRT1
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Queuine TRNA-Ribosyltransferase Catalytic Subunit 1
Introduction
QTRT1 is the catalytic subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine and tyrosine.
Entrez Gene ID
81890
UniProt ID
Q9BXR0
Alternative Names
TGT; TGUT; FP3235
Function
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154).
Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).
Biological Process
Biological Process tRNA modificationManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process tRNA-guanine transglycosylationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
Mitochondrion outer membrane
Weakly associates with mitochondria, possibly via QTRT2.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-QTRT1 Recombinant Antibody (RB50086)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quoteOnline Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry