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Mouse Anti-PRKRA Monoclonal Antibody (3A4) (CBMAB-0007-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3A4
Antibody Isotype
IgG2b
Application
IP, MA

Basic Information

Immunogen
Recombinant peptide (Full Length)
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
PRKRA
Introduction
This gene encodes a protein that belongs to a conserved family of histone methyltransferases that predominantly act as negative regulators of transcription. The encoded protein contains an N-terminal Su(var)3-9, Enhancer-of-zeste, and Trithorax (SET) domain and a double zinc-finger domain. The protein activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis.
Entrez Gene ID
8575
UniProt ID
O75569
Alternative Names
DYT16; HSD14; PACT; RAX
Function
Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity).
Biological Process
Cellular response to oxidative stressIEA:Ensembl
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Middle ear morphogenesisIEA:Ensembl
miRNA processingManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of cell population proliferationManual Assertion Based On ExperimentTAS:ProtInc
Outer ear morphogenesisIEA:Ensembl
Positive regulation of intrinsic apoptotic signaling pathwayIEA:Ensembl
Pre-miRNA processingManual Assertion Based On ExperimentIDA:BHF-UCL
Protein phosphorylationIEA:Ensembl
Protein stabilizationManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of production of small RNA involved in gene silencing by RNAManual Assertion Based On ExperimentIBA:GO_Central
Response to virusManual Assertion Based On ExperimentTAS:ProtInc
siRNA processingManual Assertion Based On ExperimentIDA:UniProtKB
Skeletal system morphogenesisIEA:Ensembl
Cellular Location
Cytoplasm, perinuclear region
Cytoplasm
Involvement in disease
Dystonia 16 (DYT16):
An early-onset dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT16 patients have progressive, generalized dystonia with axial muscle involvement, oro-mandibular (sardonic smile) and laryngeal dystonia and, in some cases, parkinsonian features.
PTM
Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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