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Mouse Anti-PASK Recombinant Antibody (8G9) (CBMAB-P0842-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
8G9
Antibody Isotype
IgG2a
Application
FC, IF, IHC-P, WB

Basic Information

Immunogen
Full length human recombinant protein of human PASK (NP_055963) produced in HEK293T cell.
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
PAS Domain Containing Serine/Threonine Kinase
Introduction
PASK is a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes.
Entrez Gene ID
23178
UniProt ID
Q96RG2
Alternative Names
PAS Domain Containing Serine/Threonine Kinase; PASKIN; PAS Domain-Containing Serine/Threonine-Protein Kinase; Per-Arnt-Sim (PAS) Domain Kinase; EC 2.7.11.1; PAS-Kinase; KIAA0135; STK37; HPASK;
Function
Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation.
Biological Process
Energy homeostasisISS:UniProtKB
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of glycogen biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of translationManual Assertion Based On ExperimentTAS:UniProtKB
Protein autophosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of glucagon secretionISS:UniProtKB
Regulation of respiratory gaseous exchangeISS:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Localizes in the nucleus of testis germ cells and in the midpiece of sperm tails.
PTM
Autophosphorylated on Thr-1161 and Thr-1165. Autophosphorylation is activated by phospholipids.
More Infomation

Xiao, M., Dhungel, S., Azad, R., Favaro, D. C., Rajesh, R. P., Gardner, K. H., & Kikani, C. K. (2024). Signal-regulated Unmasking of Nuclear Localization Motif in the PAS Domain Regulates the Nuclear Translocation of PASK. Journal of Molecular Biology, 436(3), 168433.

Xu, S., Fan, L., Zaborniak, P., Zhu, R., Ji, H., Madden, K. S., ... & Bronowska, A. K. (2024). “Druggability” of the PAS domains of human PASK kinase, a therapeutic target for metabolic and liver disorders. QRB Discovery, 1-21.

Leusenko, A. V., Mironov, K. S., & Los, D. A. (2024). Transmembrane and PAS domains of the histidine kinase Hik33 as regulators of cold and light responses in the cyanobacterium Synechocystis sp. PCC 6803. Biochimie, 218, 76-84.

Zhu, R. (2023). Computational structure-based investigation of factors governing the biological function of protein PAS domains (Doctoral dissertation, Newcastle University).

Hurtado-Carneiro, V., Dongil, P., Pérez-García, A., Álvarez, E., & Sanz, C. (2021). Preventing oxidative stress in the liver: an opportunity for glp-1 and/or pask. Antioxidants, 10(12), 2028.

Dongil, P., Pérez-García, A., Hurtado-Carneiro, V., Herrero-de-Dios, C., Álvarez, E., & Sanz, C. (2020). PAS kinase deficiency reduces aging effects in mice. Aging (Albany NY), 12(3), 2275.

Karakkat, J. V., Kaimala, S., Sreedharan, S. P., Jayaprakash, P., Adeghate, E. A., Ansari, S. A., ... & Starling Emerald, B. (2019). The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex. Nucleic acids research, 47(19), 10086-10103.

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For research use only. Not intended for any clinical use.

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