Mouse Anti-PAFAH1B2 Recombinant Antibody (CBYC-P133) (CBMAB-P0658-YC)

Name: Mouse Anti-PAFAH1B2 Recombinant Antibody (CBYC-P133)
SKU: CBMAB-P0658-YC
Rating: 5 (1 reviews)

See all PAFAH1B2 antibodies

Online Inquiry

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYC-P133

Antibody Isotype

IgG2a, κ

Application

ELISA

Basic Information

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.2

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Platelet Activating Factor Acetylhydrolase 1b Catalytic Subunit 2

Introduction

Platelet-activating factor acetylhydrolase (PAFAH) inactivates platelet-activating factor (PAF) into acetate and LYSO-PAF. This gene encodes the beta subunit of PAFAH, the other subunits are alpha and gamma. Multiple alternatively spliced transcript variants have been described for this gene.

Entrez Gene ID

5049

UniProt ID

P68402

Alternative Names

HSD-PLA2

Function

Alpha2 catalytic subunit of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)) heterotetrameric enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and modulates the action of PAF. The activity and substrate specificity of PAF-AH (I) are affected by its subunit composition. The alpha2/alpha2 homodimer (PAFAH1B2/PAFAH1B2 homodimer) hydrolyzes PAF and 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylethanolamine (AAGPE) more efficiently than 1-O-alkyl-2-acetyl-sn-glycero-3-phosphoric acid (AAGPA). In contrast, the alpha1/alpha2 heterodimer(PAFAH1B3/PAFAH1B3 heterodimer) hydrolyzes AAGPA more efficiently than PAF, but has little hydrolytic activity towards AAGPE (By similarity).
May play a role in male germ cell meiosis during the late pachytenestage and meiotic divisions as well as early spermiogenesis (By similarity).

Biological Process

Brain developmentManual Assertion Based On ExperimentIBA:GO_Central
Lipid catabolic processIEA:UniProtKB-KW
Lipid metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of macroautophagyManual Assertion Based On ExperimentIMP:BHF-UCL
SpermatogenesisISS:UniProtKB

Cellular Location

Cytoplasm

More Infomation

References

Tanigawa, K., Kiriya, M., Hayashi, Y., Shinden, Y., Kijima, Y., Natsugoe, S., ... & Karasawa, K. (2022). Cathepsin G-induced malignant progression of MCF-7 cells involves suppression of PAF signaling through induced expression of PAFAH1B2. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1867(8), 159164.

Xu, Z. Y., Peng, J., Shi, Z. Z., Chen, X. L., Cheng, H. Z., Wang, H., ... & Peng, H. (2021). Silencing linc00662 inhibits cell proliferation and colony formation of lung cancer cells via regulating the miR-145-5p–PAFAH1B2 axis. Biochemistry and Cell Biology, 99(3), 330-338.

Kim, G. R., Zhao, T., Kee, H. J., Kee, S. J., & Jeong, M. H. (2021). MicroRNA-212-5p and its target PAFAH1B2 suppress vascular proliferation and contraction via the downregulation of RhoA. Plos one, 16(3), e0249146.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-PAFAH1B2 Recombinant Antibody (CBYC-P133)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-PAFAH1B2 Recombinant Antibody (2F4-1C10)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: