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Mouse Anti-OTUB1 Recombinant Antibody (5F6) (CBMAB-O0705-CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
5F6
Antibody Isotype
IgG1
Application
FC, IF, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
OTUB1
Introduction
The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
55611
UniProt ID
Q96FW1
Alternative Names
OTU Deubiquitinase, Ubiquitin Aldehyde Binding 1; OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 1; Ubiquitin-Specific-Processing Protease OTUB1; OTU Domain, Ubiquitin Aldehyde Binding 1; Deubiquitinating Enzyme OTUB1; Otubain-1; OTB1; OTU1
Function
Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.
Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain.
Biological Process
Adaptive immune responseIEA:UniProtKB-KW
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
DNA repairIEA:UniProtKB-KW
Negative regulation of double-strand break repairManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of histone H2A K63-linked ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein K48-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
More Infomation

Zhao, Y., Ruan, J., Li, Z., Su, X., Chen, K., Lin, Y., ... & Wang, X. (2023). OTUB1 inhibits breast cancer by non‐canonically stabilizing CCN6. Clinical and Translational Medicine, 13(8), e1385.

Liao, Y., Yang, M., Wang, K., Wang, Y., Zhong, B., & Jiang, N. (2022). Deubiquitinating enzyme OTUB1 in immunity and cancer: good player or bad actor?. Cancer letters, 526, 248-258.

Lu, L., Zhai, X., Li, X., Wang, S., Zhang, L., Wang, L., ... & Wang, F. (2022). Met1-specific motifs conserved in OTUB subfamily of green plants enable rice OTUB1 to hydrolyse Met1 ubiquitin chains. Nature Communications, 13(1), 4672.

Han, X., Ren, C., Lu, C., Qiao, P., Yang, T., & Yu, Z. (2022). Deubiquitination of MYC by OTUB1 contributes to HK2 mediated glycolysis and breast tumorigenesis. Cell Death & Differentiation, 29(9), 1864-1873.

Yan, C., Yang, H., Su, P., Li, X., Li, Z., Wang, D., ... & Ding, Y. (2022). OTUB1 suppresses Hippo signaling via modulating YAP protein in gastric cancer. Oncogene, 41(48), 5186-5198.

Zhang, H. H., Li, C., Ren, J. W., Liu, L., Du, X. H., Gao, J., ... & Li, S. Z. (2021). OTUB1 facilitates bladder cancer progression by stabilizing ATF6 in response to endoplasmic reticulum stress. Cancer Science, 112(6), 2199-2209.

Koschel, J., Nishanth, G., Just, S., Harit, K., Kröger, A., Deckert, M., ... & Schlüter, D. (2021). OTUB1 prevents lethal hepatocyte necroptosis through stabilization of c-IAP1 during murine liver inflammation. Cell Death & Differentiation, 28(7), 2257-2275.

Zhou, X., Yu, J., Cheng, X., Zhao, B., Manyam, G. C., Zhang, L., ... & Sun, S. C. (2019). The deubiquitinase Otub1 controls the activation of CD8+ T cells and NK cells by regulating IL-15-mediated priming. Nature immunology, 20(7), 879-889.

Liu, T., Jiang, L., Tavana, O., & Gu, W. (2019). The deubiquitylase OTUB1 mediates ferroptosis via stabilization of SLC7A11. Cancer research, 79(8), 1913-1924.

Li, Y., Yang, J. Y., Xie, X., Jie, Z., Zhang, L., Shi, J., ... & Sun, S. C. (2019). Preventing abnormal NF-κB activation and autoimmunity by Otub1-mediated p100 stabilization. Cell research, 29(6), 474-485.

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For research use only. Not intended for any clinical use.

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