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Mouse Anti-NUDT1 Recombinant Antibody (AT3B3) (CBMAB-N3864-WJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
AT3B3
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Saline, pH 7.4, 10% Glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Nudix Hydrolase 1
Introduction
Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Jul 2008]
Entrez Gene ID
4521
UniProt ID
P36639
Alternative Names
Nudix Hydrolase 1; Nudix Motif 1; 8-Oxo-DGTPase; Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 1; Nucleoside Diphosphate-Linked Moiety X-Type Motif 1; 8-Oxo-7,8-Dihydrodeoxyguanosine Triphosphatase; Nucleoside Diphosphate-Linked Moiety X Motif 1; 8-Oxo-7,8-Dihydroguanosine Triphosphatase;
Function
Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool (PubMed:8226881, PubMed:7713500, PubMed:10608900, PubMed:12857738, PubMed:22556419, PubMed:26238318, PubMed:24695224, PubMed:24695225, PubMed:28679043).
Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP (PubMed:10373420).
Has also a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP (PubMed:10373420, PubMed:11139615).
Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A (PubMed:8226881, PubMed:10373420, PubMed:10608900, PubMed:11756418, PubMed:12857738, PubMed:16607562, PubMed:24695224, PubMed:24695225, PubMed:26999531, PubMed:28035004).
Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA (PubMed:30304478, PubMed:32144205).
Through this antimutagenic activity protects cells from oxidative stress (PubMed:8226881, PubMed:7713500, PubMed:10608900, PubMed:12857738, PubMed:24695224, PubMed:24695225, PubMed:30304478, PubMed:32144205).
Biological Process
AgingIEA:Ensembl
DNA protectionManual Assertion Based On ExperimentIDA:UniProtKB
DNA repair1 PublicationIC:UniProtKB
Male gonad developmentIEA:Ensembl
Purine nucleoside catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Response to cadmium ionIEA:Ensembl
Response to oxidative stressManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Isoform p18
Cytoplasm, cytosol
Mitochondrion matrix
Nucleus
Mostly present in cytosol (PubMed:7782328).
A minor proportion is mitochondrial (PubMed:7782328).
A very small amount of the protein is associated with nuclei (PubMed:7782328).
Isoform p26
Mitochondrion matrix
PTM
The N-terminus is blocked.
More Infomation

Lau, H. C., Yuan, X., Huang, H., Zhang, M., Hsueh, C. Y., & Gong, H. (2023). Fusobacterium nucleatum facilitates proliferation and autophagy by activating miR-361-3p/NUDT1 axis through oxidative stress in hypopharyngeal squamous cell carcinoma. BMC cancer, 23(1), 990.

Lin, Y., Zhang, F., Jin, Y., Zhong, Q., Tan, W., Liu, J., & Wu, Z. (2022). NUDT1 could be a prognostic biomarker and correlated with immune infiltration in clear cell renal cell carcinoma. Applied bionics and biomechanics, 2022.

Huang, B., Lyu, Z., Qian, Q., Chen, Y., Zhang, J., Li, B., ... & Ma, X. (2022). NUDT1 promotes the accumulation and longevity of CD103+ TRM cells in primary biliary cholangitis. Journal of hepatology, 77(5), 1311-1324.

Shi, J., Xiong, Z., Wang, K., Yuan, C., Huang, Y., Xiao, W., ... & Zhang, X. (2021). HIF2α promotes tumour growth in clear cell renal cell carcinoma by increasing the expression of NUDT1 to reduce oxidative stress. Clinical and translational medicine, 11(11), e592.

Bialkowski, K., & Szpila, A. (2021). Specific 8-oxo-dGTPase activity of MTH1 (NUDT1) protein as a quantitative marker and prognostic factor in human colorectal cancer. Free Radical Biology and Medicine, 176, 257-264.

Vitry, G., Paulin, R., Grobs, Y., Lampron, M. C., Shimauchi, T., Lemay, S. E., ... & Bonnet, S. (2021). Oxidized DNA precursors cleanup by NUDT1 contributes to vascular remodeling in pulmonary arterial hypertension. American Journal of Respiratory and Critical Care Medicine, 203(5), 614-627.

Agarwal, S., & de Jesus Perez, V. A. (2021). In defense of the Nucleus: NUDT1 and oxidative DNA damage in pulmonary arterial Hypertension. American Journal of Respiratory and Critical Care Medicine, 203(5), 541-542.

Shen, Y., Zhang, L., Piao, S., Li, L., Li, J., Xia, Y., ... & Saiyin, W. (2020). NUDT1: A potential independent predictor for the prognosis of patients with oral squamous cell carcinoma. Journal of Oral Pathology & Medicine, 49(3), 210-218.

Bialkowski, K., & Kasprzak, K. S. (2020). A profile of 8-oxo-dGTPase activities in the NCI-60 human cancer panel: meta-analytic insight into the regulation and role of MTH1 (NUDT1) gene expression in carcinogenesis. Free Radical Biology and Medicine, 148, 1-21.

Tahara, Y. K., Kietrys, A. M., Hebenbrock, M., Lee, Y., Wilson, D. L., & Kool, E. T. (2019). Dual inhibitors of 8-oxoguanine surveillance by OGG1 and NUDT1. ACS chemical biology, 14(12), 2606-2615.

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For research use only. Not intended for any clinical use.

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