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Mouse Anti-NR2C1 Recombinant Antibody (CBYJT-1506) (CBMAB-T0540-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-1506
Antibody Isotype
IgG2b
Application
WB, IP, ELISA

Basic Information

Immunogen
Recombinant human TR2/NR2C1, aa 4-110
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 4-110

Target

Full Name
Nuclear Receptor Subfamily 2 Group C Member 1
Introduction
NR2C1 is a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. NR2C1 also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes.
Entrez Gene ID
7181
UniProt ID
P13056
Alternative Names
Nuclear Receptor Subfamily 2 Group C Member 1; Orphan Nuclear Receptor TR2; TR2; Nuclear Receptor Subfamily 2, Group C, Member 1; Nuclear Receptor Subfamily 2, Group C Isoform; TR2 Nuclear Hormone Receptor; Testicular Receptor 2
Function
Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation.
Biological Process
Anatomical structure developmentManual Assertion Based On ExperimentIBA:GO_Central
Cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIPI:UniProtKB
Positive regulation of retinoic acid receptor signaling pathwayIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Nucleus, PML body
Recruited by HDAC3, after all-trans retinoic acid stimulated MAPK1-mediated Thr-223 phosphorylation, to PML bodies for subsequent sumoylation.
PTM
Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression (By similarity).
Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-222, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-581 by PKC is important for protein stability and function as activator of RARB (By similarity).
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For research use only. Not intended for any clinical use.

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