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Mouse Anti-NLRP12 Recombinant Antibody (3B5) (CBMAB-N2683-WJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3B5
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NLR Family Pyrin Domain Containing 12
Introduction
This gene encodes a member of the CATERPILLER family of cytoplasmic proteins. The encoded protein, which contains an N-terminal pyrin domain, a NACHT domain, a NACHT-associated domain, and a C-terminus leucine-rich repeat region, functions as an attenuating factor of inflammation by suppressing inflammatory responses in activated monocytes. Mutations in this gene cause familial cold autoinflammatory syndrome type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
Entrez Gene ID
91662
UniProt ID
P59046
Alternative Names
NLR Family Pyrin Domain Containing 12; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 12; PYRIN-Containing APAF1-Like Protein 7; Regulated By Nitric Oxide; PYPAF7; NALP12; RNO; NACHT, Leucine Rich Repeat And PYD Containing 12;
Function
Plays an essential role as an potent mitigator of inflammation (PubMed:30559449).
Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways (PubMed:15489334, PubMed:17947705).
Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway (PubMed:30559449).
In turn, promotes bacterial tolerance (PubMed:30559449).
Inhibits also the DDX58-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked DDX58 activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked DDX58 degradation (PubMed:30902577).
Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue (By similarity).
Biological Process
Activation of cysteine-type endopeptidase activity involved in apoptotic process1 PublicationNAS:UniProtKB
Cellular response to cytokine stimulusManual Assertion Based On ExperimentIBA:GO_Central
Dendritic cell migrationIEA:Ensembl
Negative regulation of cytokine productionManual Assertion Based On ExperimentIDA:HGNC-UCL
Negative regulation of ERK1 and ERK2 cascadeIEA:Ensembl
Negative regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIDA:HGNC-UCL
Negative regulation of inflammatory responseManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of interleukin-1 productionManual Assertion Based On ExperimentIDA:HGNC-UCL
Negative regulation of interleukin-6 productionManual Assertion Based On ExperimentIDA:HGNC-UCL
Negative regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of protein autophosphorylationManual Assertion Based On ExperimentIDA:HGNC-UCL
Negative regulation of signal transductionManual Assertion Based On ExperimentIDA:HGNC-UCL
Negative regulation of Toll signaling pathwayManual Assertion Based On ExperimentIDA:HGNC-UCL
Positive regulation of inflammatory response1 PublicationNAS:UniProtKB
Positive regulation of interleukin-1 beta production1 PublicationNAS:UniProtKB
Positive regulation of MHC class I biosynthetic processManual Assertion Based On ExperimentIDA:MGI
Positive regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIDA:MGI
Regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIDA:MGI
Regulation of interleukin-18 production1 PublicationNAS:UniProtKB
Signal transduction1 PublicationNAS:UniProtKB
Cellular Location
Cytoplasm
Involvement in disease
Familial cold autoinflammatory syndrome 2 (FCAS2):
A rare autosomal dominant systemic inflammatory disease characterized by recurrent episodes of maculopapular rash associated with arthralgias, myalgias, fever and chills, swelling of the extremities, and conjunctivitis after generalized exposure to cold.
More Infomation

Coombs, J. R., Zamoshnikova, A., Holley, C. L., Maddugoda, M. P., Teo, D. E. T., Chauvin, C., ... & Schroder, K. (2024). NLRP12 interacts with NLRP3 to block the activation of the human NLRP3 inflammasome. Science Signaling, 17(820), eabg8145.

Huang, L., Tao, Y., Wu, X., Wu, J., Shen, M., & Zheng, Z. (2023). The role of NLRP12 in inflammatory diseases. European Journal of Pharmacology, 175995.

Khan, S., Kwak, Y. T., Peng, L., Hu, S., Cantarel, B. L., Lewis, C. M., ... & Zaki, H. (2023). NLRP12 downregulates the Wnt/β-catenin pathway via interaction with STK38 to suppress colorectal cancer. The Journal of Clinical Investigation, 133(19).

Tsao, Y. P., Tseng, F. Y., Chao, C. W., Chen, M. H., Yeh, Y. C., Abdulkareem, B. O., ... & Chen, S. T. (2023). NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression. The Journal of clinical investigation, 133(3).

Tweedell, R. E., & Kanneganti, T. D. (2023). NLRP12‐PANoptosome in haemolytic, infectious and inflammatory diseases. Clinical and Translational Medicine, 13(9).

Chen, H., Deng, Y., Gan, X., Li, Y., Huang, W., Lu, L., ... & Chi, W. (2020). NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma. Molecular Neurodegeneration, 15(1), 1-16.

Prado, D. S., Veras, F. P., Ferreira, R. G., Damasceno, L. E. A., Melo, P. H., Zamboni, D. S., ... & Alves‐Filho, J. C. (2020). NLRP12 controls arthritis severity by acting as a checkpoint inhibitor of Th17 cell differentiation. The FASEB Journal, 34(8), 10907-10919.

Tuladhar, S., & Kanneganti, T. D. (2020). NLRP12 in innate immunity and inflammation. Molecular aspects of medicine, 76, 100887.

Del Porto, F., Cifani, N., Proietta, M., Verrecchia, E., Di Rosa, R., Manna, R., & Chiurazzi, P. (2020). NLRP12 gene mutations and auto-inflammatory diseases: ever-changing evidence. Rheumatology, 59(11), 3129-3136.

Chen, S. T., Chen, L., Lin, D. S. C., Chen, S. Y., Tsao, Y. P., Guo, H., ... & Ting, J. P. Y. (2019). NLRP12 regulates anti-viral RIG-I activation via interaction with TRIM25. Cell host & microbe, 25(4), 602-616.

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For research use only. Not intended for any clinical use.

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