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Mouse Anti-NFE2 Recombinant Antibody (A1333) (CBMAB-AP12590LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
A1333
Antibody Isotype
IgG1
Application
ELISA, WB

Basic Information

Immunogen
Fusion Protein of NFE2
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
Affinity purity
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Nuclear Factor, Erythroid 2
Introduction
Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
Entrez Gene ID
4778
UniProt ID
Q16621
Alternative Names
Nuclear Factor, Erythroid 2; Nuclear Factor, Erythroid-Derived 2 45 KDa Subunit; Nuclear Factor (Erythroid-Derived 2), 45kDa; Nuclear Factor (Erythroid-Derived 2), 45kD; Leucine Zipper Protein NF-E2; P45 NF-E2; Transcription Factor NF-E2 45 KDa Subunit; NF-E2; P45;
Function
Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
Biological Process
Hemostasis Source: ProtInc
Integrated stress response signaling Source: ComplexPortal
Multicellular organism development Source: ProtInc
Nucleosome disassembly Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
PML body
Cytoplasm
Note: The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs). Translocated to the cytoplasm through interaction with ITCH.
PTM
Phosphorylated on serine residues. In undifferentiated erythrocytes, phosphorylated by MAPK8 which then leads to ubiquitination and protein degradation.
Sumoylated. Sumoylation is required for translocation to nuclear bodies PODs, anchoring to the gene loci, and transactivation of the beta-globin gene.
Ubiquitinated mainly by 'Lys63'-linked ubiquitin. Polyubiquitination with 'Lys63'-linked ubiquitin by ITCH retains NFE2 in the cytoplasm preventing its transactivation activity. In undifferentiated erythrocyte, ubiquitinated after MAPK8-mediatd phosphorylation leading to protein degradation (By similarity).
More Infomation

Łoboda, A., & Dulak, J. (2023). Nuclear Factor Erythroid 2-Related Factor 2 and Its Targets in Skeletal Muscle Repair and Regeneration. Antioxidants & Redox Signaling, 38(7-9), 619-642.

Ho, D. V., Suryajaya, K. G., Manh, K., Duong, A. N., & Chan, J. Y. (2023). Characterization of NFE2L1-616, an isoform of nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes. Scientific Reports, 13(1), 19900.

Basu, P., Averitt, D. L., Maier, C., & Basu, A. (2022). The effects of nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2) activation in preclinical models of peripheral neuropathic pain. Antioxidants, 11(2), 430.

Dou, R., Wang, X., & Zhang, J. (2022). Prognostic value and immune infiltration analysis of nuclear factor erythroid-2 family members in ovarian cancer. BioMed Research International, 2022.

D'Orazi, G., Garufi, A., & Cirone, M. (2020). Nuclear factor erythroid 2 (NF‐E2) p45‐related factor 2 interferes with homeodomain‐interacting protein kinase 2/p53 activity to impair solid tumors chemosensitivity. IUBMB life, 72(8), 1634-1639.

Smolková, K., Mikó, E., Kovács, T., Leguina-Ruzzi, A., Sipos, A., & Bai, P. (2020). Nuclear factor erythroid 2-related factor 2 in regulating cancer metabolism. Antioxidants & Redox Signaling, 33(13), 966-997.

Zeng, Z., Wang, Z. Y., Li, Y. K., Ye, D. M., Zeng, J., Hu, J. L., ... & Li, Z. H. (2020). Nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) in non-small cell lung cancer. Life sciences, 254, 117325.

Zhou, J., Zhang, X., Tang, H., Yu, J., Zu, X., Xie, Z., ... & Lei, X. (2020). Nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) in autophagy-induced hepatocellular carcinoma. Clinica Chimica Acta, 506, 1-8.

Paramasivan, P., Kankia, I. H., Langdon, S. P., & Deeni, Y. Y. (2019). Emerging role of nuclear factor erythroid 2-related factor 2 in the mechanism of action and resistance to anticancer therapies. Cancer Drug Resistance, 2(3), 490.

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For research use only. Not intended for any clinical use.

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