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Mouse Anti-NEK6 Recombinant Antibody (4B10) (CBMAB-N1938-WJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
4B10
Antibody Isotype
IgG2b, κ
Application
ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NIMA (never in mitosis gene a)-related kinase 6
Introduction
The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Entrez Gene ID
10783
UniProt ID
Q9HC98
Alternative Names
NIMA Related Kinase 6; NIMA (Never In Mitosis Gene A)-Related Kinase 6; Putative Serine-Threonine Protein Kinase; Never In Mitosis A-Related Kinase 6; NimA-Related Protein Kinase 6; Protein Kinase SID6-1512; EC 2.7.11.1; Serine/Threonine-Protein Kinase Nek6; SID6-1512;
Function
Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:14563848).

Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis (PubMed:19414596).

Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3 (PubMed:12054534, PubMed:20873783).

Phosphorylates KIF11 to promote mitotic spindle formation (PubMed:19001501).

Involved in G2/M phase cell cycle arrest induced by DNA damage (PubMed:18728393).

Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (PubMed:21099361).

Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757).
Biological Process
Apoptotic process Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Chromosome segregation Source: UniProtKB
Mitotic nuclear membrane disassembly Source: Reactome
Mitotic spindle organization Source: Reactome
Peptidyl-serine phosphorylation Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of cellular senescence Source: UniProtKB
Regulation of mitotic cell cycle Source: UniProtKB
Regulation of mitotic metaphase/anaphase transition Source: UniProtKB
Spindle assembly Source: UniProtKB
Cellular Location
Nucleus
Nucleus speckle
Cytoplasm
Cytoskeleton
centrosome
spindle pole
Note: Colocalizes with APBB1 at the nuclear speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis.
PTM
Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210.
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For research use only. Not intended for any clinical use.

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