Mouse Anti-MSH3 Recombinant Antibody (CBFYM-2623) (CBMAB-M2811-FY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYM-2623

Antibody Isotype

IgG1

Application

WB, IF

Basic Information

Immunogen

Human MSH3 aa. 136-349

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Aqueous buffered solution containing BSA, glycerol

Preservative

0.09% sodium azide

Concentration

0.25 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 136-349

Target

Full Name

MUTS HOMOLOG 3

Introduction

The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer.

Entrez Gene ID

4437

UniProt ID

P20585

Alternative Names

MutS Homolog 3; Divergent Upstream Protein; Mismatch Repair Protein 1; HMSH3; MRP1; DUP; DNA Mismatch Repair Protein Msh3

Function

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Biological Process

DNA repair Source: BHF-UCL
Maintenance of DNA repeat elements Source: HGNC-UCL
Mismatch repair Source: ComplexPortal
Mitotic recombination Source: GO_Central
Negative regulation of DNA recombination Source: BHF-UCL
Positive regulation of helicase activity Source: BHF-UCL
Somatic recombination of immunoglobulin gene segments Source: GO_Central

Cellular Location

Nucleus
MutSbeta complex
nucleoplasm
nucleus
Other locations
membrane

Involvement in disease

Endometrial cancer (ENDMC):
A malignancy of endometrium, the mucous lining of the uterus. Most endometrial cancers are adenocarcinomas, cancers that begin in cells that make and release mucus and other fluids.
Familial adenomatous polyposis 4 (FAP4):
A form of familial adenomatous polyposis, a condition characterized by the development of multiple colorectal adenomatous polyps, benign neoplasms derived from glandular epithelium. Some affected individuals may develop colorectal carcinoma. FAP4 inheritance is autosomal recessive.

More Infomation

References

Koi, M., Leach, B. H., McGee, S., Tseng-Rogenski, S. S., Burke, C. A., & Carethers, J. M. (2024). Compound heterozygous MSH3 germline variants and associated tumor somatic DNA mismatch repair dysfunction. NPJ Precision Oncology, 8(1), 12.

Oh, J. M., Kang, Y., Park, J., Sung, Y., Kim, D., Seo, Y., ... & Myung, K. (2023). MSH2-MSH3 promotes DNA end resection during homologous recombination and blocks polymerase theta-mediated end-joining through interaction with SMARCAD1 and EXO1. Nucleic Acids Research, 51(11), 5584-5602.

Lamb, N. A., Bard, J. E., Loll-Krippleber, R., Brown, G. W., & Surtees, J. A. (2022). Complex mutation profiles in mismatch repair and ribonucleotide reductase mutants reveal novel repair substrate specificity of MutS homolog (MSH) complexes. Genetics, 221(4), iyac092.

Ambati, B. K., Varshney, A., Lundstrom, K., Palú, G., Uhal, B. D., Uversky, V. N., & Brufsky, A. M. (2022). MSH3 homology and potential recombination link to SARS-CoV-2 furin cleavage site. Frontiers in Virology, 2, 10.

Perne, C., Peters, S., Cartolano, M., Horpaopan, S., Grimm, C., Altmüller, J., ... & Aretz, S. (2021). Variant profiling of colorectal adenomas from three patients of two families with MSH3-related adenomatous polyposis. Plos one, 16(11), e0259185.

AbdelGawwad, M. R., Marić, A., Al-Ghamdi, A. A., & Hatamleh, A. A. (2019). Interactome analysis and docking sites of MutS homologs reveal new physiological roles in Arabidopsis thaliana. Molecules, 24(13), 2493.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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