Mouse Anti-MPG (AA 1-90) Recombinant Antibody (CBFYM-2485) (CBMAB-M2672-FY)

Name: Mouse Anti-MPG (AA 1-90) Recombinant Antibody (CBFYM-2485)
SKU: CBMAB-M2672-FY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYM-2485

Antibody Isotype

IgG2a, k

Application

ELISA, IF, IHC-P, WB

Basic Information

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MPARSGAQFC RRMGQKKQRP ARAGQPHSSS DAAQAPAEQP HSSSDAAQAP CPRERCLGPP TTPGPYRSIY FSSPKGHLTR LGLEFFDQPA

Specificity

Human

Antibody Isotype

IgG2a, k

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-90

Target

Full Name

N-methylpurine-DNA glycosylase

Introduction

MPG is a Protein Coding gene. Among its related pathways are Telomere C-strand Synthesis and Depurination. Gene Ontology annotations related to this gene include damaged DNA binding and DNA-3-methyladenine glycosylase activity.

Entrez Gene ID

4350

UniProt ID

P29372

Alternative Names

N-Methylpurine DNA Glycosylase; 3-Methyladenine DNA Glycosidase; 3-Alkyladenine DNA Glycosylase; Mid1; Anpg; ADPG; AAG; 3 End Of The Mid1 Gene, Localized 68 Kb Upstream The Humanzeta Globin Gene On 16p; CRA36.1 (3-Methyl-Adenine DNA Glycosylase); N-Methylpurine-DNA Glycosylase, MPG; Proliferation-Inducing Protein 11

Function

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

Biological Process

Base-excision repair Source: GO_Central
Depurination Source: Reactome
DNA dealkylation involved in DNA repair Source: ProtInc

Cellular Location

Mitochondrion
mitochondrion nucleoid
Cytoplasm
Nucleus

More Infomation

References

Nguyen, T. T., Rajakannu, P., Pham, M. D. T., Weman, L., Jucht, A., Buri, M. C., ... & Hegi, M. E. (2023). Epigenetic silencing of HTATIP2 in glioblastoma contributes to treatment resistance by enhancing nuclear translocation of the DNA repair protein MPG. Molecular Oncology, 17(9), 1744-1762.

Tong, H., Wang, X., Liu, Y., Liu, N., Li, Y., Luo, J., ... & Yang, H. (2023). Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase. Nature Biotechnology, 1-5.

Wu, X., Ren, B., Liu, L., Qiu, S., Li, P., Yan, F., ... & Zhou, H. (2023). Adenine base editor incorporating the N-methylpurine DNA glycosylase MPGv3 enables efficient A-to-K base editing in rice. Plant Communications, 4(6).

Hill, P., Zellmann, F., Vukova, T., Marini, F., Kolmar, S., Kaina, B., ... & Nikolova, T. (2022). Dose response to methylating agents in the γH2AX, SCE and colony formation assays: Effect of MGMT and MPG overexpression. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 876, 503462.

Hegi, M., Nguyen, T. T., Rajakannu, P., Pham, M. D. T., Weman, L., Jucht, A., ... & Van Dommelen, K. (2022). Epigenetic silencing of HTATIP2 in glioblastoma enhances nuclear translocation of the DNA-repair protein MPG affecting treatment resistance.

Mechetin, G. V., Endutkin, A. V., Diatlova, E. A., & Zharkov, D. O. (2020). Inhibitors of DNA glycosylases as prospective drugs. International Journal of Molecular Sciences, 21(9), 3118.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

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SDS
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