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Mouse Anti-MICAL1 Recombinant Antibody (3D12) (CBMAB-A5481-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3D12
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
MICAL1 (NP_073602.2, 971 a.a. ~ 1067 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Microtubule Associated Monooxygenase, Calponin And LIM Domain Containing 1
Entrez Gene ID
64780
UniProt ID
Q8TDZ2
Alternative Names
DKFZp434B1517; FLJ11937; FLJ21739; MICAL; MICAL-1; NICAL
Function
Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:29343822).

In the absence of actin, it also functions as a NADPH oxidase producing H2O2 (PubMed:21864500, PubMed:26845023, PubMed:29343822).

Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels (By similarity).

May act as Rab effector protein and play a role in vesicle trafficking.
Biological Process
Actin filament bundle assembly Source: GO_Central
Actin filament depolymerization Source: UniProtKB
Cytoskeleton organization Source: UniProtKB
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
Negative regulation of protein phosphorylation Source: Ensembl
Regulation of regulated secretory pathway Source: Ensembl
Signal transduction Source: UniProtKB
Sulfur oxidation Source: UniProtKB
Cellular Location
Cytoskeleton
Cytoplasm
Other locations
Midbody
Note: Accumulates transiently at the abscission site before abscission occurs.
More Infomation

McGarry, D. J., Castino, G., Lilla, S., Carnet, A., Kelly, L., Micovic, K., ... & Olson, M. F. (2022). MICAL1 activation by PAK1 mediates actin filament disassembly. Cell Reports, 41(1).

Gu, H., Li, Y., Cui, X., Cao, H., Hou, Z., Ti, Y., ... & Wen, P. (2022). MICAL1 inhibits colorectal cancer cell migration and proliferation by regulating the EGR1/β-catenin signaling pathway. Biochemical Pharmacology, 195, 114870.

Yang, Y., Ye, F., Xia, T., Wang, Q., & Du, J. (2022). High MICAL1 expression correlates with cancer progression and immune infiltration in renal clear cell carcinoma. BMC cancer, 22(1), 1355.

Haikazian, S., & Olson, M. F. (2022). MICAL1 monooxygenase in autosomal dominant lateral temporal epilepsy: Role in cytoskeletal regulation and relation to cancer. Genes, 13(5), 715.

Cai, K., Deng, L., Zheng, D., Li, L., He, Z., & Yu, C. (2022). MICAL1 facilitates pancreatic cancer proliferation, migration, and invasion by activating WNT/β-catenin pathway. Journal of Translational Medicine, 20(1), 528.

McGarry, D. J., Armstrong, G., Castino, G., Mason, S., Clark, W., Shaw, R., ... & Olson, M. F. (2021). MICAL1 regulates actin cytoskeleton organization, directional cell migration and the growth of human breast cancer cells as orthotopic xenograft tumours. Cancer Letters, 519, 226-236.

Wioland, H., Frémont, S., Guichard, B., Echard, A., Jégou, A., & Romet‐Lemonne, G. (2021). Actin filament oxidation by MICAL1 suppresses protections from cofilin‐induced disassembly. EMBO reports, 22(2), e50965.

Niu, F., Sun, K., Wei, W., Yu, C., & Wei, Z. (2020). F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis. Science advances, 6(45), eabb1307.

Konstantinidis, K., Bezzerides, V. J., Lai, L., Isbell, H. M., Wei, A. C., Wu, Y., ... & Anderson, M. E. (2020). MICAL1 constrains cardiac stress responses and protects against disease by oxidizing CaMKII. The Journal of clinical investigation, 130(9), 4663-4678.

Zhao, S., Min, P., Liu, L., Zhang, L., Zhang, Y., Wang, Y., ... & Gu, L. (2019). NEDD9 facilitates hypoxia-induced gastric cancer cell migration via MICAL1 related Rac1 activation. Frontiers in pharmacology, 10, 291.

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For research use only. Not intended for any clinical use.

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