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Mouse Anti-MFN1 (AA 209-469) Recombinant Antibody (CBFYM-2154) (CBMAB-M2336-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-2154
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 209-469 of human MFN1 (NP_284941) produced in E. coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
5.45 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 209-469

Target

Full Name
mitofusin 1
Introduction
The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion. They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting.
Entrez Gene ID
55669
UniProt ID
Q8IWA4
Alternative Names
Mitofusin 1; Transmembrane GTPase MFN1; Fzo Homolog; Mitochondrial Transmembrane GTPase FZO-2; Mitochondrial Transmembrane GTPase Fzo-1; Putative Transmembrane GTPase
Function
Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12475957, PubMed:12759376, PubMed:27920125, PubMed:28114303).

Membrane clustering requires GTPase activity (PubMed:27920125).

It may involve a major rearrangement of the coiled coil domains (PubMed:27920125, PubMed:28114303).

Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12475957, PubMed:12759376).

Overexpression induces the formation of mitochondrial networks (in vitro) (PubMed:12759376).

Has low GTPase activity (PubMed:27920125, PubMed:28114303).
Biological Process
GTP metabolic process Source: UniProtKB
Mitochondrial fusion Source: BHF-UCL
Mitochondrial membrane fusion Source: UniProtKB
Mitochondrion localization Source: UniProtKB
Positive regulation of mitochondrial membrane potential Source: Ensembl
Cellular Location
Mitochondrion outer membrane
Isoform 2:
Cytoplasm
Topology
Cytoplasmic: 1-584
Helical: 585-605
Mitochondrial intermembrane: 606-608
Helical: 609-629
Cytoplasmic: 630-741
PTM
Ubiquitinated by non-degradative ubiquitin by PRKN (PubMed:23933751). Deubiquitination by USP30 inhibits mitochondrial fusion (By similarity). Ubiquitinated by MARCHF5 (PubMed:20103533). When mitochondria are depolarized and dysfunctional, it is ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that contains FBXO7 and PRKN (PubMed:23933751).
More Infomation

Stavropoulos, F., Georgiou, E., Schiza, N., Bell, S., Baloh, R. H., Kleopa, K. A., & Sargiannidou, I. (2023). Mitofusin 1 overexpression rescues the abnormal mitochondrial dynamics caused by the Mitofusin 2 K357T mutation in vitro. Journal of the Peripheral Nervous System.

Huang, K., Pei, S., Sun, Y., Xu, X., Fang, Y., Lai, M., ... & Zheng, X. (2023). Mitofusin 1-Mediated Redistribution of Mitochondrial Antiviral Signaling Protein Promotes Type 1 Interferon Response in Human Cytomegalovirus Infection. Microbiology Spectrum, 11(2), e04615-22.

Kırmızıgül, Ö. A., Sabanci, A., Dişli, F., Yıldız, S., Milward, M. R., & Aral, K. (2023). Evaluation of the role of mitofusin‐1 and mitofusin‐2 in periodontal disease. Journal of Periodontology.

Sidarala, V., Zhu, J., Levi-D’Ancona, E., Pearson, G. L., Reck, E. C., Walker, E. M., ... & Soleimanpour, S. A. (2022). Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis. Nature Communications, 13(1), 2340.

Huo, Y., Sun, W., Shi, T., Gao, S., & Zhuang, M. (2022). The MFN1 and MFN2 mitofusins promote clustering between mitochondria and peroxisomes. Communications Biology, 5(1), 423.

Georgiadou, E., Muralidharan, C., Martinez, M., Chabosseau, P., Akalestou, E., Tomas, A., ... & Rutter, G. A. (2022). Mitofusins Mfn1 and Mfn2 are required to preserve glucose-but not incretin-stimulated β-cell connectivity and insulin secretion. Diabetes, 71(7), 1472-1489.

Dorn II, G. W. (2020). Mitofusins as mitochondrial anchors and tethers. Journal of molecular and cellular cardiology, 142, 146-153.

Carvalho, K. F., Machado, T. S., Garcia, B. M., Zangirolamo, A. F., Macabelli, C. H., Sugiyama, F. H., ... & Chiaratti, M. R. (2020). Mitofusin 1 is required for oocyte growth and communication with follicular somatic cells. The FASEB Journal, 34(6), 7644-7660.

Sloat, S. R., Whitley, B. N., Engelhart, E. A., & Hoppins, S. (2019). Identification of a mitofusin specificity region that confers unique activities to Mfn1 and Mfn2. Molecular Biology of the Cell, 30(17), 2309-2319.

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For research use only. Not intended for any clinical use.

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