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Mouse Anti-MFGE8 (AA 24-387) Recombinant Antibody (CBFYM-0104) (CBMAB-M0117-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-0104
Antibody Isotype
IgG2a
Application
FC

Basic Information

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MFG-E8, Leu24-Cys387
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 24-387

Target

Full Name
Milk Fat Globule-EGF Factor 8 Protein
Introduction
This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
4240
UniProt ID
Q08431
Alternative Names
Milk Fat Globule-EGF Factor 8 Protein; Breast Epithelial Antigen BA46; Sperm Associated Antigen 10; Sperm Surface Protein HP47; Lactadherin; MFG-E8; HMFG; MFGM; SED1; O-Acetyl Disialoganglioside Synthase
Function
Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity).

Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.

Medin:
Main constituent of aortic medial amyloid.
Biological Process
Angiogenesis Source: UniProtKB-KW
Apoptotic cell clearance Source: Ensembl
Cell adhesion Source: UniProtKB-KW
Phagocytosis, engulfment Source: Ensembl
Phagocytosis, recognition Source: Ensembl
Positive regulation of phagocytosis Source: Ensembl
Single fertilization Source: UniProtKB-KW
Cellular Location
Secreted
Other locations
Membrane
acrosome membrane
Note: Located in the acrosomal region of zona-pellucida bound sperm.
PTM
Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
More Infomation

Liu, B., Zhang, B., Qi, J., Zhou, H., Tan, L., Huang, J., ... & Guan, X. Y. (2023). Targeting MFGE8 secreted by cancer-associated fibroblasts blocks angiogenesis and metastasis in esophageal squamous cell carcinoma. Proceedings of the National Academy of Sciences, 120(42), e2307914120.

Ruotsalainen, S. E., Surakka, I., Mars, N., Karjalainen, J., Kurki, M., Kanai, M., ... & Southerington, T. (2022). Inframe insertion and splice site variants in MFGE8 associate with protection against coronary atherosclerosis. Communications biology, 5(1), 802.

Wang, J., Wang, Y., Zuo, Y., Duan, J., Pan, A., Li, J. M., ... & Liu, F. (2021). MFGE8 mitigates brain injury in a rat model of SAH by maintaining vascular endothelial integrity via TIGβ5/PI3K/CXCL12 signaling. Experimental Brain Research, 239(7), 2193-2205.

Tian, J., Wang, V., Wang, N., Khadang, B., Boudreault, J., Bakdounes, K., ... & Lebrun, J. J. (2021). Identification of MFGE8 and KLK5/7 as mediators of breast tumorigenesis and resistance to COX-2 inhibition. Breast Cancer Research, 23(1), 1-18.

Lim, H., Yang, T., Lee, W., & Park, S. G. (2021). TGF-β Increases MFGE8 production in myeloid-derived suppressor cells to promote B16F10 melanoma metastasis. Biomedicines, 9(8), 896.

Wang, B., Ge, Z., Wu, Y., Zha, Y., Zhang, X., Yan, Y., & Xie, Y. (2020). MFGE8 is down‐regulated in cardiac fibrosis and attenuates endothelial‐mesenchymal transition through Smad2/3‐Snail signalling pathway. Journal of cellular and molecular medicine, 24(21), 12799-12812.

Ni, Y. Q., Zhan, J. K., & Liu, Y. S. (2020). Roles and mechanisms of MFG-E8 in vascular aging-related diseases. Ageing Research Reviews, 64, 101176.

Ge, Z., Chen, Y., Wang, B., Zhang, X., Yan, Y., Zhou, L., ... & Xie, Y. (2020). MFGE8 attenuates Ang-II-induced atrial fibrosis and vulnerability to atrial fibrillation through inhibition of TGF-β1/Smad2/3 pathway. Journal of molecular and cellular cardiology, 139, 164-175.

Cheyuo, C., Aziz, M., & Wang, P. (2019). Neurogenesis in neurodegenerative diseases: role of MFG-E8. Frontiers in neuroscience, 13, 569.

Soubeyrand, S., Nikpay, M., Turner, A., Dang, A. T., Herfkens, M., Lau, P., & McPherson, R. (2019). Regulation of MFGE8 by the intergenic coronary artery disease locus on 15q26. 1. Atherosclerosis, 284, 11-17.

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For research use only. Not intended for any clinical use.

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