Mouse Anti-MCM6 (AA 1-821) Recombinant Antibody (CBFYM-1908) (CBMAB-M2080-FY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYM-1908

Antibody Isotype

IgG2a, k

Application

ELISA, IF, WB

Basic Information

Immunogen

Full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MDLAAAAEPG AGSQHLEVRD EVAEKCQKLF LDFLEEFQSS DGEIKYLQLA EELIRPERNT LVVSFVDLEQ FNQQLSTTIQ EEFYRVYPYL CRALKTFVKD RKEIPLAKDF YVAFQDLPTR HKIRELTSSR IGLLTRISGQ V

Specificity

Human

Antibody Isotype

IgG2a, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-821

Target

Full Name

minichromosome maintenance complex component 6

Introduction

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood.

Entrez Gene ID

4175

UniProt ID

Q14566

Alternative Names

Minichromosome Maintenance Complex Component 6; Minichromosome Maintenance Deficient (Mis5, S. Pombe) 6; P105MCM; MCM6 Minichromosome Maintenance Deficient 6 (MIS5 Homolog, S. Pombe) (S. Cerevisiae); MCM6 Minichromosome Maintenance Deficient 6 (MIS5 Homolog, S. Pombe); Minichromosome Maintenance Deficient 6 Homolog (S. Cerevisiae)

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Biological Process

DNA replication Source: UniProtKB
DNA replication initiation Source: InterPro
DNA unwinding involved in DNA replication Source: ComplexPortal
Double-strand break repair via break-induced replication Source: GO_Central
Mitotic DNA replication Source: GO_Central
Regulation of DNA-dependent DNA replication initiation Source: ComplexPortal

Cellular Location

Nucleus
Other locations
Chromosome
Note: Binds to chromatin during G1 and detach from it during S phase.

PTM

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

More Infomation

References

Zhang, X., Bian, S., Ni, Y., Zhou, L., Yang, C., Zhang, C., ... & Zheng, W. (2023). Minichromosome maintenance protein family member 6 mediates hepatocellular carcinoma progression by recruiting UBE3A to induce P53 ubiquitination. International Journal of Biological Macromolecules, 248, 125854.

Shekhar, S., Verma, S., Gupta, M. K., Roy, S. S., Kaur, I., Krishnamachari, A., & Dhar, S. K. (2023). Genome-wide binding sites of Plasmodium falciparum mini chromosome maintenance protein MCM6 show new insights into parasite DNA replication. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1870(7), 119546.

Li, X., Ren, Z., Xiong, C., Geng, J., Li, Y., Liu, C., ... & Liu, H. (2020). Minichromosome maintenance 6 complex component identified by bioinformatics analysis and experimental validation in esophageal squamous cell carcinoma. Oncology Reports, 44(3), 987-1002.

Hendricks, A., Gieseler, F., Nazzal, S., Bräsen, J. H., Lucius, R., Sipos, B., ... & Schrader, C. (2019). Prognostic relevance of topoisomerase II α and minichromosome maintenance protein 6 expression in colorectal cancer. BMC cancer, 19(1), 1-10.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-MCM6 Recombinant Antibody (7D8)

Mouse Anti-MCM6 (AA 670-792) Recombinant Antibody (1/MCM6)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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