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Mouse Anti-MBD1 Recombinant Antibody (CBFYM-1798) (CBMAB-M1966-FY)


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Summary

Host Animal
Mouse
Specificity
Mouse
Clone
CBFYM-1798
Antibody Isotype
IgG1
Application
WB, ELISA

Basic Information

Immunogen
Recombinant fragment
Specificity
Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
MBD1
Introduction
The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain. These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Entrez Gene ID
17190
UniProt ID
Q9Z2E2
Alternative Names
Methyl-CpG Binding Domain Protein 1; Protein Containing Methyl-CpG-Binding Domain 1; CXXC-Type Zinc Finger Protein 3; CXXC3; PCM1; The Regulator Of Fibroblast Growth Factor 2 (FGF-2) Transcription
Function
Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
Biological Process
Negative regulation of transcription, DNA-templated Source: UniProtKB
Transcription by RNA polymerase II Source: ProtInc
Cellular Location
Nucleus
Nucleus matrix
Nucleus speckle
Other locations
Chromosome
Note: Nuclear, in a punctate pattern (PubMed:12711603). Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes (PubMed:10648624, PubMed:10454587).
PTM
Sumoylated, sumoylation may increase interaction with ATF7IP.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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