Mouse Anti-MAP3K12 (AA 828-859) Recombinant Antibody (CBFYM-1511) (CBMAB-M1674-FY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse

Clone

CBFYM-1511

Antibody Isotype

IgG1

Application

Basic Information

Immunogen

KLH conjugated synthetic peptide between 828-859 amino acids from human MAP3K12

Specificity

Human, Mouse

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 828-859

Target

Full Name

MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 12

Introduction

This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.

Entrez Gene ID

Human	7786
Mouse	26404

UniProt ID

Human	Q12852
Mouse	Q60700

Alternative Names

Mitogen-Activated Protein Kinase Kinase Kinase 12; Dual Leucine Zipper Bearing Kinase; Dual Leucine Zipper Kinase DLK; Mixed Lineage Kinase; MAPK-Upstream Kinase; EC 2.7.11.25; DLK; ZPK

Function

Part of a non-canonical MAPK signaling pathway (PubMed:28111074).
Activated by APOE, enhances the AP-1-mediated transcription of APP, via a MAP kinase signal transduction pathway composed of MAP2K7 and MAPK1/ERK2 and MAPK3/ERK1 (PubMed:28111074).
May be an activator of the JNK/SAPK pathway.

Biological Process

Histone phosphorylationISS:UniProtKB
Intracellular signal transductionManual Assertion Based On ExperimentTAS:ProtInc
JNK cascadeBy SimilarityISS:UniProtKB
Negative regulation of motor neuron apoptotic processIEA:Ensembl
Peptidyl-serine phosphorylationISS:UniProtKB
Peptidyl-threonine phosphorylationISS:UniProtKB
Positive regulation of ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of JUN kinase activityISS:ARUK-UCL
Positive regulation of protein kinase activityISS:ARUK-UCL
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Protein autophosphorylationBy SimilarityISS:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB

PTM

Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated (By similarity).
The activity of MAP3K12 can be regulated through its proteasomal degradation. APOE, through a receptor-mediated mechanism, activates MAP3K12 by preventing its proteasomal degradation.

More Infomation

References

Tenenbaum, M., Plaisance, V., Boutry, R., Pawlowski, V., Jacovetti, C., Sanchez-Parra, C., ... & Abderrahmani, A. (2021). The Map3k12 (Dlk)/JNK3 signaling pathway is required for pancreatic beta-cell proliferation during postnatal development. Cellular and Molecular Life Sciences, 78, 287-298.

Wan, W., Liu, G., Li, X., Liu, Y., Wang, Y., Pan, H., & Hu, J. (2021). MiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease. Bioengineered, 12(2), 12678-12690.

Wang, C., Wan, H., Li, M., & Zhang, C. (2021). Celastrol attenuates ischemia/reperfusion-mediated memory dysfunction by downregulating AK005401/MAP3K12. Phytomedicine, 82, 153441.

Ochieng, J. K., Kundu, S. T., Bajaj, R., Leticia Rodriguez, B., Fradette, J. J., & Gibbons, D. L. (2020). MBIP (MAP3K12 binding inhibitory protein) drives NSCLC metastasis by JNK-dependent activation of MMPs. Oncogene, 39(43), 6719-6732.

Shi, Q., Yu, J., Li, P. A., Lu, X., & Wang, W. (2020). MicroRNA-337 participates in the progression of early-stage diabetic retinopathy by upregulating MAP3K12. Minerva Medica.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-MAP3K12 Monoclonal Antibody (MM0514-9D8)

Mouse Anti-MAP3K12 Monoclonal Antibody (3A10)

Mouse Anti-MAP3K12 Monoclonal Antibody (9D8)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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