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Rabbit Anti-MAP2K4 Recombinant Antibody (5C10) (CBMAB-CP2388-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey, Dog
Clone
5C10
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human SEK1.
Specificity
Human, Mouse, Rat, Monkey, Dog
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
mitogen-activated protein kinase kinase 4
Introduction
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Entrez Gene ID
Human6416
Mouse26398
Rat287398
Monkey718155
Dog489508
UniProt ID
HumanP45985
MouseP47809
RatF1LP57
MonkeyA0A2K5WTN8
DogF1PBB6
Alternative Names
Mitogen-Activated Protein Kinase Kinase 4; Stress-Activated Protein Kinase Kinase 1; C-Jun N-Terminal Kinase Kinase 1; JNK-Activating Kinase 1; MAP Kinase Kinase 4; MAPK/ERK Kinase 4; SAPK/ERK Kinase 1; EC 2.7.12.2; MAPKK 4; SAPKK-1; PRKMK4; SAPKK1; MEK 4;
Function
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to pro-inflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.
Biological Process
Apoptotic processIEA:UniProtKB-KW
Cellular response to mechanical stimulusManual Assertion Based On ExperimentIEP:UniProtKB
Cellular senescenceTAS:Reactome
Fc-epsilon receptor signaling pathwayTAS:Reactome
JNK cascadeTAS:Reactome
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of motor neuron apoptotic processIEA:Ensembl
Positive regulation of smooth muscle cell apoptotic processIEA:Ensembl
Response to woundingIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Nucleus
PTM
Activated by phosphorylation on Ser-257 and Thr-261 by MAP kinase kinase kinases (MAP3Ks).
More Infomation

McNamara, B., Harold, J., Manavella, D., Bellone, S., Mutlu, L., Hartwich, T. M. P., ... & Santin, A. D. (2023). Uterine leiomyosarcomas harboring MAP2K4 gene amplification are sensitive in vivo to PLX8725, a novel MAP2K4 inhibitor. Gynecologic Oncology, 172, 65-71.

Wang, B., Yang, S., Jia, Y., Yang, J., Du, K., Luo, Y., ... & Zhu, B. (2022). PCAT19 regulates the proliferation and apoptosis of lung cancer cells by inhibiting miR-25-3p via targeting the MAP2K4 signal axis. Disease Markers, 2022.

Liu, X. D., Zhang, Z. W., Wu, H. W., & Liang, Z. Y. (2021). A new prognosis prediction model combining TNM stage with MAP2K4 and JNK in postoperative pancreatic cancer patients. Pathology-Research and Practice, 217, 153313.

Matsumoto, T., Yamano, A., Murakawa, Y., Fukada, H., Sawa, M., & Kinoshita, T. (2020). Ensemble structural analyses depict the regulatory mechanism of non-phosphorylated human MAP2K4. Biochemical and Biophysical Research Communications, 521(1), 106-112.

Chou, A. H., Liao, C. C., Lee, H. C., Liou, J. T., & Liu, F. C. (2019). The MAP2K4/JNK/c-Jun Signaling Pathway Plays A Key Role In Dexmedetomidine Protection Against Acetaminophen-Induced Liver Toxicity. Drug Design, Development and Therapy, 3887-3898.

Niu, J., Li, Z., & Li, F. (2019). Overexpressed microRNA-136 works as a cancer suppressor in gallbladder cancer through suppression of JNK signaling pathway via inhibition of MAP2K4. American Journal of Physiology-Gastrointestinal and Liver Physiology, 317(5), G670-G681.

Liu, S., Huang, J., Zhang, Y., Liu, Y., Zuo, S., & Li, R. (2019). MAP2K4 interacts with Vimentin to activate the PI3K/AKT pathway and promotes breast cancer pathogenesis. Aging (Albany NY), 11(22), 10697.

Lu, J., Zhou, N., Yang, P., Deng, L., & Liu, G. (2019). MicroRNA-27a-3p downregulation inhibits inflammatory response and hippocampal neuronal cell apoptosis by upregulating mitogen-activated protein kinase 4 (MAP2K4) expression in epilepsy: in vivo and in vitro studies. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 25, 8499.

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For research use only. Not intended for any clinical use.

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