Mouse Anti-MAGEA2 (AA 1-314) Recombinant Antibody (CBFYM-1305) (CBMAB-M1464-FY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYM-1305

Antibody Isotype

IgG2a, k

Application

ELISA, IF, WB

Basic Information

Immunogen

Full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MPLEQRSQHC KPEEGLEARG EALGLVGAQA PATEEQQTAS SSSTLVEVTL GEVPAADSPS PPHSPQGASS FSTTINYTLW RQSDEGSSNQ EEEGPRMFPD LESEFQAAIS RKMVELVHFL LLKYQAREPV TKAEMLESVL R

Specificity

Human

Antibody Isotype

IgG2a, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-314

Target

Full Name

melanoma antigen family A, 2

Introduction

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene.

Entrez Gene ID

4101

UniProt ID

P43356

Alternative Names

MAGE Family Member A2; Cancer/Testis Antigen Family 1, Member 2; Melanoma Antigen Family A2; Cancer/Testis Antigen 1.2; MAGE-2 Antigen; MAGEA2A; MAGE2

Function

Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. Negatively regulates acetylation and sumoylation of PML and represses PML-induced p53/TP53 acetylation and activation.

Biological Process

Cellular protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Cellular senescenceManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of protein acetylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of protein sumoylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of ubiquitin-protein transferase activityManual Assertion Based On ExperimentIMP:UniProtKB
Signal transduction by p53 class mediatorManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus
Nucleus, PML body

More Infomation

References

Bakhshi, P., Nourizadeh, M., Sharifi, L., Farajollahi, M. M., & Mohsenzadegan, M. (2023). Development of dendritic cell loaded MAGE-A2 long peptide; a potential target for tumor-specific T cell-mediated prostate cancer immunotherapy. Cancer Cell International, 23(1), 270.

Khalvandi, A., Abolhasani, M., Madjd, Z., Sharifi, L., Bakhshi, P., & Mohsenzadegan, M. (2021). Reduced cytoplasmic expression of MAGE-A2 predicts tumor aggressiveness and survival: an immunohistochemical analysis. World Journal of Urology, 39, 1831-1843.

Park, S., Han, J. E., Kim, H. G., Kim, H. Y., Kim, M. G., Park, J. K., ... & Choi, S. K. (2020). Inhibition of MAGEA2 regulates pluripotency, proliferation, apoptosis, and differentiation in mouse embryonic stem cells. Journal of Cellular Biochemistry, 121(11), 4667-4679.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-MAGEA2 Recombinant Antibody (1B1)

Mouse Anti-MAGEA2 (AA 1-314) Recombinant Antibody (CBFYM-1306)

Mouse Anti-MAGEA2 Recombinant Antibody (1H4)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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