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Mouse Anti-MAFF (AA 1-110) Recombinant Antibody (CBFYM-1283) (CBMAB-M1442-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-1283
Antibody Isotype
IgG2a, k
Application
ELISA, WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MSVDPLSSKA LKIKRELSEN TPHLSDEALM GLSVRELNRH LRGLSAEEVT RLKQRRRTLK NRGYAASCRV KRVCQKEELQ KQKSELEREV DKLARENAAM RLELDALRGK
Specificity
Human
Antibody Isotype
IgG2a, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-110

Target

Full Name
MAFF
Introduction
The protein encoded by this gene is a basic leucine zipper transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene.
Entrez Gene ID
23764
UniProt ID
Q9ULX9
Alternative Names
MAF BZIP Transcription Factor F; V-Maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Homolog F; U-MAF; V-Maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Family Protein F; V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog F; Transcription Factor MafF; HMafF
Function
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:8932385).
However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2L1/NRF1, and recruiting them to specific DNA-binding sites. Interacts with the upstream promoter region of the oxytocin receptor gene (PubMed:8932385, PubMed:16549056).
May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition (PubMed:10527846).
Biological Process
In utero embryonic developmentIEA:Ensembl
ParturitionManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:NTNU_SB
Regulation of epidermal cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Skeletal muscle cell differentiationIEA:Ensembl
Cellular Location
Nucleus
More Infomation

Bauer, S., Eigenmann, J., Zhao, Y., Fleig, J., Hawe, J. S., Pan, C., ... & von Scheidt, M. (2022). Identification of the Transcription Factor ATF3 as a Direct and Indirect Regulator of the LDLR. Metabolites, 12(9), 840.

Ibrahim, M. K., Abdelhafez, T. H., Takeuchi, J. S., Wakae, K., Sugiyama, M., Tsuge, M., ... & Wakita, T. (2021). MafF is an antiviral host factor that suppresses transcription from Hepatitis B Virus core promoter. Journal of Virology, 95(15), 10-1128.

von Scheidt, M., Zhao, Y., de Aguiar Vallim, T. Q., Che, N., Wierer, M., Seldin, M. M., ... & Schunkert, H. (2021). The transcription factor MAFF regulates an atherosclerosis relevant network connecting inflammation and cholesterol metabolism. Circulation, 143(18), 1809.

Wu, M., Deng, X., Zhong, Y., Hu, L., Zhang, X., Liang, Y., ... & Ye, X. (2020). MafF is regulated via the circ-ITCH/miR-224-5p axis and acts as a tumor suppressor in hepatocellular carcinoma. Oncology Research, 28(3), 299.

Wang, X., Zhang, Y., Wan, X., Guo, C., Cui, J., Sun, J., & Li, L. (2020). Responsive expression of MafF to β-amyloid-induced oxidative stress. Disease Markers, 2020.

Shen, S., Yang, C., Liu, X., Zheng, J., Liu, Y., Liu, L., ... & Xue, Y. (2020). RBFOX1 regulates the permeability of the blood-tumor barrier via the LINC00673/MAFF pathway. Molecular Therapy-Oncolytics, 17, 138-152.

Saliba, J., Coutaud, B., Solovieva, V., Lu, F., & Blank, V. (2019). Regulation of CXCL 1 chemokine and CSF 3 cytokine levels in myometrial cells by the MAFF transcription factor. Journal of cellular and molecular medicine, 23(4), 2517-2525.

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For research use only. Not intended for any clinical use.

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